Efficiency of single-nucleotide polymorphism haplotype estimation from pooled DNA |
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Authors: | Yang Yaning Zhang Jingshan Hoh Josephine Matsuda Fumihiko Xu Peng Lathrop Mark Ott Jurg |
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Institution: | Laboratory of Statistical Genetics, The Rockefeller University, New York, NY 10021, USA. yyang@linkage.rockefeller.edu |
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Abstract: | The efficiency of single-nucleotide polymorphism haplotype analysis may be increased by DNA pooling, which can dramatically reduce the number of genotyping assays. We develop a method for obtaining maximum likelihood estimates of haplotype frequencies for different pool sizes, assess the accuracy of these estimates, and show that pooling DNA samples is efficient in estimating haplotype frequencies. Although pooling K individuals increases ambiguities, at least for small pool size K and small numbers of loci, the uncertainty of estimation increases
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