| ESTABLISHMENT OF A HUMAN PANCREATIC ADENOCARCINOMA CELL LINE （JF305） WITH p53 EXPRESSION |
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| 引用本文： | 李昕,王宏,姜奕,王晓华,贾兰玲,张宝庚. ESTABLISHMENT OF A HUMAN PANCREATIC ADENOCARCINOMA CELL LINE （JF305） WITH p53 EXPRESSION[J]. 中国癌症研究, 1995, 7(4): 251-254. DOI: 10.1007/BF02672786 |
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| 作者姓名： | 李昕 王宏 姜奕 王晓华 贾兰玲 张宝庚 |
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| 作者单位： | Cancer Research Institute，China Medical University，Shenyang 110001 |
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| 摘 要： | 李昕，王宏，姜奕，王晓华，贾兰玲，张宝庚ＥＳＴＡＢＬＩＳＨＭＥＮＴＯＦＡＨＵＭＡＮＰＡＮＣＲＥＡＴＩＣＡＤＥＮＯＣＡＲＣＩＮＯＭＡＣＥＬＬＬＩＮＥ（ＪＦ３０５）ＷＩＴＨｐ５３ＥＸＰＲＥＳＳＩＯＮ￥ＬｉＸｉｎ；ＷａｎｇＨｏｎｇ；ＪｉａｎｇＹｉ；Ｗａｎｇ...
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| 修稿时间： | 1995-05-25 |
Establishment of a human pancreatic adenocarcinoma cell line (JF305) with p53 expression |
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| Li Xin,Wang Hong,Jiang Yi,Wang Xiaohua,Jia Lanling,Zhang Baogeng. Establishment of a human pancreatic adenocarcinoma cell line (JF305) with p53 expression[J]. Chinese Journal of Cancer Research, 1995, 7(4): 251-254. DOI: 10.1007/BF02672786 |
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| Authors: | Li Xin Wang Hong Jiang Yi Wang Xiaohua Jia Lanling Zhang Baogeng |
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| Affiliation: | (1) Cancer Research Institute, China Medical University, 110001 Shenyang |
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| Abstract: | A human pancreatic cancer cell line (JF305) was established from a pancreatic adenocarcinoma xenograft in nude mice. These cells have passed 103 passages for more than 1 year. The plating effitiency was 30.6%, and doubling time was 49.5 hours. Chromosomal analysis showed a human karyotype with a chromosome number between 66-130. JF305 cells exhibited,epithelial morphoogic features resembling the structure of the original tumor, and they grew to form tumor when inoculated again into nude mice. With immuuohistochemical staining, the nuclei of JF305 cells were positive for P53 protein. Therefor, JF305 offers a good model for the study of human pancreatic adenocarnoma and p53 gene mutation. |
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| Keywords: | Pancreatic Tumor Gene expression Cell line |
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