多病程Wistar大鼠实验性变态反应性脑脊髓炎的病理研究

目的:在建立wistar大鼠多病程实验性变态反应性脑脊髓炎(EAE)的动物模型的基础上,进行病理学研究,探讨不同发病类型EAE的基本病理改变如炎细胞浸润、脱髓鞘和轴索损伤等方面的差别,为多发性硬化(MS)的研究提供实验依据。方法:以豚鼠全脊髓匀浆(GPSCH)为抗原免疫Wistar大鼠建立EAE的动物模型,进行常规HE染色、Weil髓鞘染色和改良的Bielschowsky,并行GFAP免疫组化染色,观察不同发病类型EAE的病理政变。结果:根据病理和临床表现可将Wistar大鼠EAE模型分为5种发病形式:急性型、缓解-复发型、持续进展型、良性型和隐匿型。光镜下可见不同发病时期的EAE的病理改变有所不同,急性型EAE炎症浸润明显,尤脱髓鞘改变,缓解-复发型和持续进展型EAE髓鞘脱失和轴索损伤更明显,且有陈旧病灶周围的星型胶质细胞增生,而新发病灶无此表现,良性型EAE则改变接近正常。结论:首次建立了Wistar大鼠多病程EAE,且病理证实不同类型EAE的炎细胞分布、髓鞘脱失及轴索损伤等基本病理改变是不同的,它具有人类MS的许多发病特点,其中多病程的发病形式和主要病理特点与MS极其相似,是理想的MS动物模型。

Research the pathological changes of the rats in multi-course EAE

Objective: On the basis of establishing the multiple clinical processes model, we explored the pathological characteristics of EAE model . such as inflammatory cells intruding, demyelinating and axon injury, to provide the experimental value for reseaching MS. Method: The animal model was established in Wistar rat by immunization with complete Frcund adjuvant and GPSCH , the pathological changes of EAE were studied with the aid of staining with hematoxylin-e osin(HE). Weil myelin staining and modified Bielschowsky staining, marking astrocytes with glial fibrillary acid protein (GFAP)with the method of immunohistochemistry (IHC) in the mean time. Results: We can divide the EAE into five types by pathological changes and clinical manifestation: acute EAE, relapsing-remitting EAE, persistent progress EAE, benign form EAE and delitescence EAE. Every type showed different characteristics, acute EAE mainly showed inflammatory infiltration and there were no demyelination, relapsing-remitting EAE and persistent progress EAE showed obviously demyelination and axon damage, there were proliferation of gliocytes around the old lesions, but there were no proliferation around the new lesions, benign form EAE were normal. Conclusion: Multiple clinical processes on EAE model of Wistar rat were established for the first time , there were different pathological changes in inflammatory infiltration , demyelination and axon damage , proliferation of gliocytes around the lesions in different course EAE, It shared many features with the MS patients, the course of the disease and the main pathological features were similar to MS, so it is an i-deal animal model, it is an ideal animal model in studying MS in our country.