高迁移率族蛋白B1多态性与肺癌化疗患者肺部感染严重程度的相关性

目的探讨高迁移率族蛋白B1(HMGB1)多态性和血清表达与肺癌化疗患者肺部感染及其严重程度的关系。方法选择2018年3月-2020年1月商丘市第一人民医院肿瘤内科收治的晚期肺癌化疗患者118例作为研究对象,根据其化疗期间是否发生肺部感染分为感染组(n=57)及非感染组(n=61)。采用聚合酶链式扩增反应(PCR)对两组患者外周血HMGB1 rs1412125(T>C)、HMGB1 rs2249825(C>G)进行基因分型,分析感染组HMGB1不同基因型患者、C-反应蛋白(CRP)、降钙素原(PCT)水平及肺部感染严重程度评分(Pneumonia severity index,PSI)。结果感染组HMGB1 rs1412125位点CC基因型、C等位基因频率, rs2249825位点GG基因型、G等位基因频率均显著高于非感染组(P<0.05);校正性别、年龄等因素后,多元Logistic回归结果显示,HMGB1 rs1412125位点CC基因型、C等位基因,rs2249825位点GG基因型、G等位基因是本地区晚期肺癌化疗患者肺部感染的危险因素;感染组患者HMGB1 rs1412125位点CC型血清HMGB1、CRP、PCT水平及PSI评分均高于TT型及TC型(P<0.05),感染组患者HMGB1 rs2249825位点GG型血清HMGB1、CRP、PCT水平及PSI评分高于CG型及CC型(P<0.05)。结论 HMGB1 rs1412125位点、HMGB1 rs2249825位点多态性与本地区肺癌化疗患者肺部感染易感性及严重程度有关,HMGB1 rs1412125位点CC型、HMGB1 rs2249825位点GG型为易感基因型。

Correlation between polymorphism of high mobility group protein B1 and severity of pulmonary infection in lung cancer patients undergoing chemotherapy

OBJECTIVE To explore the correlation between polymorphism of high mobility group protein B1(HMGB1), serum expression and severity of pulmonary infection in the lung cancer patients undergoing chemotherapy. METHODS A total of 118 advanced lung cancer patients who received chemotherapy and were treated in oncology department of Shangqiu First People′s Hospital from Mar 2018 to Jan 2020 were recruited as the study subjects and were divided into the infection group with 57 cases and the non-infection group with 61 cases according to the status of pulmonary infection during the chemotherapy.The genotyping of peripheral blood HMGB1 rs1412125(T>C) and HMGB1 rs2249825(C>G) was carried out by using polymerase chain reaction(PCR) for amplification.The genotypes of HMGB1, C-reactive protein(CRP), procalcitonin(PCT) and pneumonia severity index(PSI) of the patients with infection were observed. RESULTS The HMGB1 rs1412125 locus CC genotype, C allele frequency, rs2249825 locus GG genotype and G allele frequency of the infection group were significantly higher than those of the non-infection group(P<0.05).After the factors such as gender and age were adjusted, multivariate logistic regression analysis showed that HMGB1 rs1412125 locus CC genotype, C allele, rs2249825 locus GG genotype and G allele were the risk factors for the pulmonary infection in the advanced lung cancer patients undergoing chemotherapy.Among the patients of the infection group, the levels of serum HMGB1, CRP, PCT and PSI of the patients with HMGB1 rs1412125 locus CC genotype were significantly higher than those of the patients with TT genotypes and TC genotype(P<0.05);the levels of serum HMGB1, CRP, PCT and PSI of the patients with HMGB1 rs2249825 locus GG genotype were significantly higher than those of the patients with CG genotype and CC genotype(P<0.05). CONCLUSION The polymorphisms of HMGB1 rs1412125 locus and HMGB1 rs2249825 locus are associated with the susceptibility and severity of pulmonary infection in the lung cancer patients undergoing chemotherapy.The HMGB1 rs1412125 locus CC and HMGB1 rs2249825 locus GG are the susceptible genotypes.