杜仲叶中倍半萜及降碳倍半萜的分离与鉴定

目的研究植物杜仲(Eucommia ulmoides Oliver)叶中的化学成分。方法综合运用硅胶柱色谱、反相硅胶柱色谱和Sephadex LH-20凝胶柱色谱以及制备型高效液相色谱等方法进行系统分离,根据化合物的理化性质及其波谱数据确定化合物的结构。结果从杜仲叶80%(体积分数)乙醇提取物中分离得到了12个倍半萜和降碳倍半萜类化合物,分别鉴定为(3S,5R,6R,7E,9R)-megastigman-7-ene-3,5,6,9-tetrol(1)、(3S,5R,6R,7E,9S)-megastigman-7-ene-3,5,6,9-tetrol(2)、(3R,5R,6R,7E)-3,5,6-trihydroxy-7-megastigmen-9-one(3)、(3S,5R,6R,7E)-3,5,6-trihydroxy-7-megastigmen-9-one(4)、cis-3,4-dihydroxy-β-ionon(5)、3-hydroxy-5,6-epoxy-β-ionol(6)、(S)-3-hydroxy-8-ionone(7)、(6R,9S)-9,10-dihydroxy-4-megastigmen-3-one(8)、cucumegastigmane I(9)、(-)-菜豆酸(10)、(-)-2E-菜豆酸(11)、蚱蜢酮(12)。结论化合物11为新化合物,2~10和12为首次从杜仲中分离得到。

Isolation and identification of sesquiterpenoids and norisoprenoids from leaves of Eucommia ulmoides Oliver

To study the chemical composition of leaves of Eucommia ulmoides Oliver,twelve compounds were separated and purified by silica gel chromatography,ODS column chromatography,Sephadex LH-20 chromatography and HPLC methods.Their structures were identified on the basis of physical and chemical properties and spectroscopic data(1H-NMR,13C-NMR,MS) as(3S,5R,6R,7E,9R)-megastigman-7-ene-3,5,6,9-tetrol(1),(3S,5R,6R,7E,9S)-megastigman-7-ene-3,5,6,9-tetrol(2),(3R,5R,6R,7E)-3,5,6-trihydroxy-7-megastigmen-9-one(3),(3S,5R,6R,7E)-3,5,6-trihydroxy-7-megastigmen-9-one(4),cis-3,4-dihydroxy-β-ionon(5),3-hydroxy-5,6-epoxy-β-ionol(6),(S)-3-hydroxy-8-ionone(7),(6R,9S)-9,10-dihydroxy-4-megastigmen-3-one(8),cucumegastigmane I(9),(-)-phaseic acid(10),(-)-2E-phaseic acid(11),grasshopper ketone(12).Compound 11 was a new compound,and compounds 2-10 and 12 were isolated from the Eucommia ulmoides Oliver for the first time.