不同浓度bFGF对体外培养人牙髓细胞增殖的影响

目的：探讨不同浓度碱性成纤维细胞生长因子(bFGF) 对体外培养人牙髓细胞增殖的影响，为寻求最佳影响浓度提供参考依据。方法：以常规组织块培养法体外获得牙髓细胞,随机分为5组，实验组分别加入bFGF，使其终浓度为0.1、1.0、10.0及100.0 μg?L^-1，无bFGF组作为阴性对照。采用MTT法分别测定各组吸光度A值，计算细胞相对增殖率（RGR），观察bFGF对牙髓细胞的增殖作用。结果：人牙髓细胞在不同浓度bFGF的作用下，除0.1 μg?L^-1bFGF组外，其他实验组RGR均高于对照组 (P<0.01)；组间比较显示10.0 μg?L^-1 bFGF组RGR最高，与1.0 μg?L^-1bFGF组比较差异有显著性(P<0.01)，但与100.0 μg?L^-1bFGF 组比较差异无显著性(P>0.05)。结论：bFGF能促进人牙髓细胞的增殖，bFGF的最小显效浓度是1.0 μg?L^-1，最佳增殖浓度是10.0 μg?L^-1。  

Effect of bFGF with different doses on proliferation of human dental pulp cells in vitro

Objective To investigate the effect of different concenrtrations of basic fibroblast growth factor (bFGF) on proliferation of human dental pulp cell in vitro,and to find out the most effective concentration of bFGF. Methods Dental pulp cells were isolated from dental pulp tissue explants.The pulp cells were divided into 5 groups randomly,bFGF was added into each group until the ultimately concentrations were 0.1,1.0,10.0 and 100.0 μg·L-1respectively while the group without bFGF as control group. The effects of bFGF on dental pulp cells were assayed by absorbency A and relative growth rate(RGR) with MTT colorimetric method. Results bFGF at concentrations of 1.0-100.0 μg·L-1 promoted the cell proliferation (P<0.01). The difference between 10.0 μg·L-1 and 1.0 μg·L-1 bFGF groups was significant(P<0.01),while that was not obvious between 10.0 μg·L-1 and 100.0 μg·L-1 bFGF groups(P>0.05). Conclusion bFGF has the capability of promoting the proliferation of human dental pulp cells,and the smallest effective concentration is 1.0 μg·L-1,the most strong cell proliferation takes place at bFGF concentration of 10.0 μg·L-1.