| 生脉散对大肠杆菌脂多糖诱导慢性肝衰竭大鼠细胞因子水平的影响 |
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| 引用本文： | 徐文军,周小舟,马文峰,孙新锋,黄俏光,周大桥.生脉散对大肠杆菌脂多糖诱导慢性肝衰竭大鼠细胞因子水平的影响[J].中国医师杂志,2011,13(5):581-583. |
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| 作者姓名： | 徐文军 周小舟 马文峰 孙新锋 黄俏光 周大桥 |
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| 作者单位： | 广州中医药大学附属深圳医院国家中医肝病重点专科,深圳,518033 |
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| 基金项目： | "艾滋病和病毒性肝炎等重大传染病防治"科技重大专项"十一五"课题"慢性重型肝炎证候规律及中西医结合治疗方案研究"，国家中医药管理局留学回国人员科技择优项目，广东省深圳市科技重大项目 |
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| 摘 要： | 目的探讨生脉散对慢性肝衰竭大鼠大肠杆菌脂多糖（LPS）诱导细胞因子水平的影响。方法采用CCl。混合液腹腔注射复制慢性肝衰竭大鼠模型，观察LPS诱导2h后生脉散后对血清内毒素、细胞因子水平的影响。结果CCl。混合液能明显增加大鼠血清IL-6（64．50±18．79）pg／mlVS（4．79±0．57）pg／m1]、ICAM-1（25100．00±5258．85）pg／mlVS（4215．50±942．79）pg／m1]和TNF-α（17．55±2．39）pg／mlVS（10．92±5．02）pg／m1]水平（P〈0．05），但对血清LPS水平无明显影响（0．058±0．007）EU／mlVS（0．040±0．002）EU／ml，P〉0．05]；中药生脉散能显著降低CCl。慢性肝衰竭大鼠血清IL-6、ICAM-1和TNF-α水平分别为（17．20±3．12）pg／ml、（9490．00±2725．78）pg／ml、（3．00±1．00）pg／ml，P〈0．05]。LPS攻击2h后能明显升高CCI。混合液大鼠血清LPS、TNF-α、IL-6、ICAM-1水平分别为（0．501±0．019）EU／ml、（19750．00±9655．17）pg／ml、（5615．00±490．50）pg／ml、（41000．00±589．88）pg／ml，P〈0．01]。结论在慢性肝衰竭模型中，LPS能增加TNF-α，IL-6和ICAM-1等炎症因子水平，中药生脉散可降低LPS水平，并抑制LPS诱导的炎症因子水平，阻断了炎性介质及LPS本身对机体的损伤。
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| 关 键 词： | 生脉散／药理学 脂多糖类／副作用 肝功能衰竭／代谢／中药疗法 内毒素类／代谢 肿瘤坏死因子∥代谢 白细胞介素lβ／代谢 白细胞介素6／代谢 胞间黏附分子1／代谢 |
Effects of Sheng Mai San on the level of cell factors induced by lipopolysaccharide in chronic liver failure rats |
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| XU Wen-jun,ZHOU Xiao-zhou,MA Wen-feng,SUN Xin-feng,HUANG Qiao-guang,ZHOU Da-qiao.Effects of Sheng Mai San on the level of cell factors induced by lipopolysaccharide in chronic liver failure rats[J].Journal of Chinese Physician,2011,13(5):581-583. |
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| Authors: | XU Wen-jun ZHOU Xiao-zhou MA Wen-feng SUN Xin-feng HUANG Qiao-guang ZHOU Da-qiao |
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| Institution: | .( Liver Department of The Shenzhen Affiliated Hospital of Guangzhou College of TCM, Shenzhen 518033, China) |
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| Abstract: | Objective To study the Sheng Mai San on the levels of cell factors induced by lipopolysaccharide in acute liver failure rats. Methods The models of chronic liver failure were constructed by injecting CCl4 in the abdomen of rats. The serum levels of lipopolysaccharide and cell factors were determined after treating with LPS and Sheng Mai San for 2 hours. Results The serum level of IL-6［(64.50±18.79)pg/ml vs (4.79±0.57)pg/ml］, ICAM-1［(25100.00±5258.85)pg/ml vs (4215.50±942.79)pg/ml］ and TNF-α［(17.55±2.39)pg/ml vs (10.92±5.02)pg/ml］ was increased by CCl4 (P＜0.05), but there is no effect on the serum level of LPS in rats ［(0.058±0.007)EU/ml vs (0.040±0.002)EU/ml,P＞0.05］. Sheng Mai San can significantly reduce the serum level of IL-6, ICAM-1 and TNF-α in rats with acute liver failure induced by CCl4 ［(17.20±3.12)pg/ml,(9490.00±2725.78)pg/ml,(3.00±1.00)pg/ml,P＜0.05］. After treating with LPS for 2 hours, the serum level of LPS, TNF-α, IL-6, ICAM-1 markedly increased ［(0.501±0.019)EU/ml,(19750.00±9655.17)pg/ml,(5615.00±490.50)pg/ml,(41000.00±589.88)pg/ml,P＜0.01］. Sheng Mai San could reduce the serum levels of LPS, TNF-α, IL-6, ICAM-1 and in rats with chronic liver failure (P＜0.01). Conclusions SD Rats in the state of chronic liver fail-ure, existing serious serum endotoxin, can induce the levels of cell factors by diversification inflammation reaction and. ShengMaiSan can regulating the levels of cell factors in rats with chronic liver failure. |
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| Keywords: | SHENGMAI POWDERS/PD Lipopolysaccharides/AE Liver failure/ME/ZD Endotoxins/ME Tumor necrosis factor-alpha/ME Interleukin-1beta/ME Interleukin-6/ME Intercellular adhesion molecule-1/ME |
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