| 小鼠胡须体觉皮质局灶性脑缺血模型建立 |
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| 引用本文： | 李文磊,姜亚军,闵敏,丁新生.小鼠胡须体觉皮质局灶性脑缺血模型建立[J].中国临床神经科学,2011,19(4):357-362. |
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| 作者姓名： | 李文磊 姜亚军 闵敏 丁新生 |
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| 作者单位： | 1. 南京中医药大学附属医院江苏省中医院神经内科,210029
2. 南京医科大学第一附属医院神经内科,210029 |
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| 基金项目： | 南京中医药大学附院江苏省中医院院级课题 |
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| 摘 要： | 目的：制作梗死区集中在小鼠大脑胡须体觉皮质的局灶性脑缺血模型。方法：小鼠麻醉后,经右侧顶叶皮质处开颅,显微镜和内在光信号成像下结扎大脑中动脉分支2～3支。应用激光多普勒血流仪检测结扎前、后局部脑血流情况,TTC染色检测梗死面积大小,TUNEL染色标记细胞死亡情况,细胞色素氧化酶染色确定体觉皮质和梗死部位的关系。结果：大脑中动脉分支结扎后,梗死中心及周围局部脑血流明显下降。缺血后3 d,TTC染色示梗死区位于胡须体觉皮质,梗死面积约占脑冠状切面面积的15%,TUNEL染色示死亡细胞位于胡须体觉皮质,不累及白质,细胞色素氧化酶染色示胡须体觉皮质结构破坏。结论：可选择性将小鼠具有单一功能的胡须体觉皮质制作成局灶性脑缺血模型,为脑缺血研究的动物模型提供更多选择。
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| 关 键 词： | 局灶性脑缺血 模型 胡须体觉皮质 小鼠 |
Construction of Whisker Barrel Cortex Focal Ischemic Animal Model in Mouse |
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| LI Wen-Lei,JIANG Ya-Jun,MIN Min,DING Xin-Sheng.Construction of Whisker Barrel Cortex Focal Ischemic Animal Model in Mouse[J].Chinese Journal of Clinical Neurosciences,2011,19(4):357-362. |
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| Authors: | LI Wen-Lei JIANG Ya-Jun MIN Min DING Xin-Sheng |
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| Institution: | LI Wen-Lei1,JIANG Ya-Jun1,MIN Min1,DING Xin-Sheng2 1Department of Neurology,Jiangsu Provincial Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Traditional Chinese Medicine,2Department of Neurology,the First Affiliated Hospital of Nanjing Medical University,Nanjing,210029,China |
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| Abstract: | Aim:To set up a focal cerebral ischemia animal model that the infarct region targets at whisker barrel cortex of mice.Methods:The mice were subjected to anesthesia,and the 2~3 branches of right middle cerebral artery(MCA) supplying the barrel cortex were permanently ligated under the direction of microscope and intrinsic optical signal imaging.Before and after ligation,local cerebral blood flow was assessed by laser Doppler scanner.The infarct volume was measured by TTC staining.Cell death was assessed by T... |
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| Keywords: | focal cerebral ischemia animal model whisker barrel cortex mouse |
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