| 维胃方治疗大鼠实验性胃溃疡的尿液代谢组学研究 |
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| 引用本文： | 彭树灵,刘晓伟,张真瑞,贺红燕,杨健.维胃方治疗大鼠实验性胃溃疡的尿液代谢组学研究[J].时珍国医国药,2011,22(5). |
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| 作者姓名： | 彭树灵 刘晓伟 张真瑞 贺红燕 杨健 |
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| 作者单位： | 南方医科大学中医药学院,广东,广州,510515 |
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| 基金项目： | 国家自然科学基金，广东省中医药管理局基金，国家发明专利号:ZL200710028412.6 |
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| 摘 要： | 目的 探讨维胃方治疗大鼠实验性胃溃疡的尿液代谢表型的变化及相关机制.方法 采用乙酸灼烧法制备大鼠胃溃疡模型;运用气相色谱-质谱(GC-MS)获得正常对照组、模型组、自愈组与维胃方高、中、低剂量组的大鼠尿液代谢物谱.运用偏最小二乘法判别分析(PLS-DA),寻找表征正常对照组与模型组差异的生物标记物;使用主成分分析(PCA)考察维胃方不同剂量组对胃溃疡的治疗效果,并根据标记物水平的变化解释相关机制.结果 模型组与正常对照组的尿液代谢物谱具有显著差异;获得的生物标记物包括有机酸、氨基酸等,两组标记物水平有显著性差异(P<0.01或P<0.05).维胃方不同剂量组的物质水平出现不同程度回调,以中剂量组幅度最大.结论 胃溃疡大鼠出现代谢异常,维胃方通过调节机体的代谢机能有效治疗胃溃疡,且以中剂量为佳;运用代谢组学技术可从整体上揭示中药对疾病的治疗作用与机制.
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| 关 键 词： | 代谢组学 维胃方 胃溃疡 大鼠 |
Metabonomic Investigation of Urine from Experimental Gastric Ulcer Rats Treated with Weiweifang Recipe |
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| PENG Shu-ling,LIU Xiao-wei,ZHANG Zhen-rui,HE Hong-yan,YANG Jian.Metabonomic Investigation of Urine from Experimental Gastric Ulcer Rats Treated with Weiweifang Recipe[J].Lishizhen Medicine and Materia Medica Research,2011,22(5). |
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| Authors: | PENG Shu-ling LIU Xiao-wei ZHANG Zhen-rui HE Hong-yan YANG Jian |
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| Institution: | PENG Shu-ling,LIU Xiao-wei*,ZHANG Zhen-rui,HE Hong-yan,YANG Jian(School of Traditional Chinese Medicine,Southern Medical University,Guangzhou 510515,China) |
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| Abstract: | Objective To investigate the changes of urinary metabolic phenotype and their related mechanisms in experimental gastric ulcer rats treated with Weiweifang(WWF,a kind of Chinese medicine recipe).Methods Forty-eight male Wistar rats were randomly divided into 6 groups: control group,model group,spontaneously healing group and WWF treatment groups(high-dose,middle-dose and low-dose groups),with 8 rats in each group.Gastric ulcer was caused by serosal application of acetic acid in rats and metabolic spectra of... |
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| Keywords: | Metabonomics Weiweifang Recipe(WWF) Gastric ulcer Rat |
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