外周血单核细胞血红素加氧酶1表达与糖尿病肾病的氧化应激机制的研究

目的探讨糖尿病肾病患者外周血单核细胞（PBMC）血红素加氧酶-1（HO-1）的表达与糖尿病肾病的关系及其可能机制。方法2型糖尿病患者伴或不伴糖尿病肾病各20例，正常对照组20例。比较各组空腹血糖、糖化血红蛋白（HbAlc）等基本资料，并检测三组血清中丙二醛（MDA）含量、外周血单核细胞活性氧（ROS）水平、HO-1mRNA水平、HO-1蛋白表达水平。结果在正常对照组、糖尿病组、糖尿病肾病组中，血清MDA水平分别为（14．23±5．07）nmoL／ml、（24．90±7．12）nmol／ml、（43．83-i-16．97）nmot／ml，三组相比，差异有统计学意义（F=37．022，P〈0．01）；PBMC的ROS水平分别为113．18±58．59、364．54±88．67、524．35±162．51，三组相比，差异有统计学意义（F=68．369，P〈0．01）；HO．1蛋白表达水平分别为22．84±9．98、36．72±15．85、58．1±15．93，三组相比，差异有统计学意义（F：31．302，P〈0．01）。HO-1mRNA的水平及蛋白表达水平与MDA水平均呈正相关（r=0．407，0．429，P〈0．05）。结论糖尿病肾病患者与糖尿病非肾病患者相比处于更严重的氧化应激状态并诱导的HO-1代偿性增高。提高HO-1表达将可能是改善糖尿病肾病患者氧化应激状态的可能途径。

Expression of heme oxygenase-1 in peripheral blood mononuclear cells and mechanism of oxidative stress in diabetic nephropathy

Objective To investigate the relationship and mechanism of the heme oxygenase-1 expression in peripheral blood mononuclear cells (PBMC) and the oxidative stress in diabetic nephropathy. Methods Two groups of diabetic patients with or without diabetic nephropathy and a normal control group were enrolled in this study. Fasting blood glucose (FBG), HbA1c, serum MDA level, ROS level, HO-1 mRNA level and HO-1 protein expression in PBMC were determined. Results In control group, diabetic group and diabetic nephropathy group , the MDA levels significantly increased(14.23±5.07)nmol/ml vs (24.90±7.12)nmol/ml vs (43.83±16.97)nmol/ml](F＝37.022,P＜0.01), the ROS levels significantly increased (113.18±58.59 vs 364.54±88.67 vs 524.35±162.51)(F＝68.369,P＜0.01) and the HO-1 protein expression also increased significantly (22.84±9.98 vs 36.72±15.85 vs 58.1±15.93)(F＝31.302,P＜0.01). There was a positive correlation among the HO-1 mRNA, protein expression and MDA level(r＝0.407,0.429,P＜0.05). Conclusions There existed a severer oxidative stress condition in patients with diabetic nephropathy compared with the patients without diabetic nephropathy. HO-1 could be a potential pathway to ameliorate oxidative stress in diabetic kidney disease patients.