Encapsulation of Lactobacillus plantarum ATCC 8014 and Pediococcus acidilactici ATCC 8042 in a freeze-dried alginate-gum arabic system and its in vitro testing under gastrointestinal conditions |
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Authors: | I Sandoval-Mosqueda J F Montiel-Sosa L Corona Z Guadarrama-Álvarez |
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Institution: | 1. Laboratory of Biopreservation, Multidisciplinary Research Unit, Faculty of Higher Studies Cuautitlan, National Autonomous University of Mexico, Cuautitlan Izcalli, Mexico;2. Department of Animal Nutrition and Biochemistry, Faculty of Veterinary Medicine and Zootechnichs, National Autonomous University of Mexico, Cuautitlan Izcalli, Mexico |
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Abstract: | AbstractThe aim of this study was to investigate the viability of Pediococcus acidilactici ATCC 8042 and Lactobacillus plantarum ATCC 8014 in a freeze-dried capsules system prepared with sodium alginate and gum arabic using the extrusion technique. The capsules made with alginate 2% (w/v)/gum arabic 2% (w/v) showed higher hardness (7.12?±?0.71?N), with highly cohesive (0.81?±?0.02) and elastic (0.99?±?0.00) features on the Texture Profile Analysis (TPA), as well as higher sphericity with 1.75?±?0.12?mm y 1.73?±?0.13?mm diameter axes and regularity in their surface by Scanning Electron Microscopy (SEM). The use of skimmed milk at 10% as a cryoprotector in the freeze-drying process allowed the obtention of high viability percentages (88% a 96%) for both strains. Best results of viability for P. acidilactici encapsulated was with the use of alginate 2% (w/v)/gum arabic 2% (w/v) (92%±2.65), and L. plantarum with the use of alginate 2% (w/v) (84.71%±10.33) during the gastrointestinal environment challenge. |
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Keywords: | Encapsulation extrusion probiotics sodium alginate gum arabic freeze-drying |
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