| α-1酸性糖蛋白的分离纯化 |
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| 引用本文： | 侯一平,芶清,吴梅筠. α-1酸性糖蛋白的分离纯化[J]. 四川大学学报(医学版), 1993, 0(3) |
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| 作者姓名： | 侯一平 芶清 吴梅筠 |
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| 作者单位： | 华西医科大学法医学系血清学教研室(侯一平，芶清)，华西医科大学法医学系血清学教研室(吴梅筠) |
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| 摘 要： | 本文报道α-1酸性糖蛋白的分离纯化。人血清经DEAE-Sephadex-A50和ReactiveBlue 2-Sepharose CL-6B两次柱层析即可获得纯化的α-1酸性糖蛋白。纯化产物经聚丙烯酰胺凝胶电泳、SDS-聚丙烯酰胺梯度凝胶电泳、免疫电泳和琼脂双向扩散等证明,其纯度和特异性均符合要求。
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| 关 键 词： | α-1酸性糖蛋白 血清类粘蛋白 亲和层析 分离纯化 |
Isolation and Purification of Alpha -1 Acid Glycoprotein from Human Sera |
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| Hou Yiping,Gou Qing,Wu Meiyun. Isolation and Purification of Alpha -1 Acid Glycoprotein from Human Sera[J]. Journal of Sichuan University. Medical science edition, 1993, 0(3) |
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| Authors: | Hou Yiping Gou Qing Wu Meiyun |
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| Affiliation: | Hou Yiping,Gou Qing,Wu Meiyun Department of Serology,School of Forensic Medicine |
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| Abstract: | Alpha-1 acid glycoprotein,also named orosomucoid (ORM),is a serumprotein with genetic polymorphism. Thispaper describes a two-step procedure forpurifying ORM from human sera. The proce-dure consists of both anion exchange chro-matography on a DEAE-Sephadex-A50eolumn and affinity chromatography on aReactive Blue 2-Sepharose CL-6B column.The purified ORM identified by polyacry-lamide gel electrophoresis, SDS-polyacryla-mide gradient gel electrophoresis, immuno-electrophoresis and immunodiffusion washomogeneous and reacted specifically witha commercial anti-ORM serum (Sigma). Themolecular weight of the purified ORM wasabout 41 kd. |
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| Keywords: | Alpha-1 acid glycoprotein Orosomucoid Affinity chromatography Isolation and purification |
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