| HPLC法测定脊痛宁胶囊中蛇床子素的含量 |
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| 引用本文： | 白林,李程程. HPLC法测定脊痛宁胶囊中蛇床子素的含量[J]. 解放军药学学报, 2013, 0(6): 542-544 |
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| 作者姓名： | 白林 李程程 |
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| 作者单位： | [1]解放军总医院药品保障中心药检室,北京100853 [2]怀柔区第一医院药剂科,北京101400 |
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| 摘 要： | 目的建立脊痛宁胶囊中蛇床子素的含量测定方法。方法 HPLC法,色谱柱：Diamonsil（R）C18（250 mm×4.6 mm,5μm）;流动相：甲醇-水（68∶32）;流速：1.0 ml·min-1;检测波长：322 nm;柱温：40℃。结果蛇床子素在7.52~75.2μg·ml-1范围内线性关系良好,r=0.9999;回收率为101.30%,RSD为0.15%。结论本方法简便、准确,专属性强,测定结果重复性好,可用于脊痛宁中蛇床子素的质量控制。
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| 关 键 词： | 脊痛宁胶囊 蛇床子素 HPLC |
Determination of Osthole in Jitongning Capsules by HPLC |
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| BAI Lin,LI Cheng-cheng. Determination of Osthole in Jitongning Capsules by HPLC[J]. Pharmaceutical Journal of Chinese People's Liberation Army, 2013, 0(6): 542-544 |
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| Authors: | BAI Lin LI Cheng-cheng |
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| Affiliation: | 1.Department of Drug Analysis, Medicine Supply Center, PLA General Hospital, Beijing 100853, China ; 3.Department of Pharmacy,Huairou First Hospital,Beijing 101400,China) |
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| Abstract: | Objective To establish an HPLC method for the determination of the content of osthole in extracts of Jitongning. Methods HPLC was applied. Diamonsil（R） C 18（250 mm ×4. 6 mm,5 μm） was used as the chromatographic colum with methanol-water（68∶ 32） as the mobile phase. The flow rate was 1. 0 ml·min-1,which was determined under 322 nm. The column temperature was 40 ℃. Results The linear range of osthole was 7. 52-75. 2 μg·ml-1（r = 0. 9999） with an average recovery of 101. 30%（RSD = 0. 15%,n = 7）. Conclusion This method is simple and accurate,with good specificity and repeatability,which can serve as a scientific quantitative analysis method for Jitongning capsules. |
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| Keywords: | Jitongning capsule osthole HPLC |
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