羟基磷灰石表面修饰人工角膜钛支架的生物相容性

背景：纯钛作为安全的生物种植体材料已被用于制作俄罗斯钛人工角膜,但其临床使用中仍存在人工角膜移位、漏水、角膜组织融解或人工角膜排出等并发症。目的：观察经羟基磷灰石表面修饰人工角膜钛支架在碱烧伤兔角膜内的生物相容性。方法：将30只右眼角膜碱烧伤新西兰白兔随机分为3组,实验组于角膜基质层内植入经羟基磷灰石表面修饰的人工角膜钛支架,对照组于角膜基质层内植入人工角膜钛支架,空白对照组仅作角膜板层切口。结果与结论：所有支架在观察期内稳定存留,无角膜坏死融解及支架脱出发生。术后2,8周,实验组与对照组炎症细胞浸润数量明显高于空白对照组（P〈0.05）,16周后各组间炎症细胞浸润数量差异无显著性意义。术后2,8,16周,实验组角膜成纤维细胞数高于对照组和空白对照组（P〈0.05）。实验组支架表面有密集的角膜细胞及细胞外基质紧密抓附,支架与角膜组织愈合良好;对照组支架表面仅见少量角膜组织简单包裹,支架与角膜组织未真正愈合。表明羟基磷灰石表面修饰的人工角膜钛支架可在碱烧伤兔角膜组织中稳定存留,生物相容性较单纯钛支架提高。

Biocompatibility of hydroxyapatite modified titanium skirt for keratoprosthesis

BACKGROUND： Titanium implants as a safe biological material have been used to produce the artificial Russian titanium cornea, but complications still exist, including artificial cornea shift, leakage, corneal tissue melting and artificial cornea discharge. OBJECTIVE： To evaluate in vivo biocompatibility of hydroxyapatite modified titanium skirt for keratoprosthesis in alkali burn cornea. METHODS： A total of 30 alkali burned New Zealand white rabbit corneas were divided into three group groups. Hydroxyapatite modified titanium skirt （experimental group） and titanium skirt （control group） were respectively inserted into the corneal stroma of rabbits. In the blank control group, only a lamellar corneal incision was made. RESULTS AND CONCLUSION： All skirts were stable without necrosis, melting and exclusion during the observation period. The number of inflammatory cells in the experimental and control groups was significantly higher than that in the blank control group at 2 and 8 weeks postoperatively （P 〈 0.05）, but there was no difference in inflammatory cell infiltration among different groups by the 16th week. The number of corneal fibroblasts increased significantly in the experimental group compared with the control and blank control group after 2, 8, 16 weeks （P 〈 0.05）. The extracellular matrix deposited on the surface of hydroxyapatite modified titanium skirt was denser and tighter than that on the surface of titanium skirt. It indicates that hydroxyapatite modified titanium skirt for keratoprosthesis can promote the interfacial biointegration of skirt and host cornea.