纳米钛酸钙对鼠成骨细胞增殖与分化的影响

背景：以不同修饰剂制备的纳米钛酸钙具有良好的骨传导性、生物相容性及生物活性。目的：观察纳米钛酸钙材料对成骨细胞增殖、分化的影响。方法：分别以聚乙二醇、十六烷基三甲基溴化铵、柠檬酸钠为修饰剂制备纳米钛酸钙,同时制备无修饰剂的纳米钛酸钙。将制备的4组纳米钛酸钙分别配制为0.1,1.0,10 g/L的浸提液,MTT方法检测材料浸提液对乳鼠成骨细胞增殖及碱性磷酸酶活性的影响,以正常培养的细胞为对照。结果与结论：以聚乙二醇、十六烷基三甲基溴化铵、柠檬酸钠为修饰剂制备的纳米钛酸钙,无修饰剂的纳米钛酸钙在1.0,10 g/L质量浓度下可明显促进成骨细胞的增殖;上述4组纳米钛酸钙浸提液在1.0,10 g/L质量浓度下,培养3,6,9 d的细胞A值也高于对照组（P〈0.05,P〈0.01）,培养6,9 d的细胞碱性磷酸酶活性值均高于对照组（P〈0.05,P〈0.01）。表明以聚乙二醇、十六烷基三甲基溴化铵、柠檬酸钠、无修饰剂制备的钛酸钙对成骨细胞无毒性作用,可促进成骨细胞的增殖和分化。

Nano-calcium titanate effects on rat osteoblast proliferation and differentiation

BACKGROUND： Nano-calcium titanate, prepared with different modifiers, have properties of good osteogenesis, bone conductibility and biocompatibility. OBJECTIVE： To observe the. influence of nano-calcium titanate materials on the proliferation and differentiation of osteoblasts. METHODS： Nano-calcium titanate was prepared with modifiers such as polyethylene glycol, acetyl trim ethyl ammonium bromide and sodium citrate. Meanwhile, nano-calcium titanate without modifier was prepared. All the four kinds of nano-calcium titanate materials were used to prepare nano-calcium titanate extracts under the concentration of 0.1, 1.0, 10 g/L, respectively. The proliferation of osteoblasts was observed through 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide assay and alkaline phosphatase activity of osteoblasts was observed. Osteoblasts cultured in normal condition served as contrast. RESULTS AND CONCLUSION： The proliferation of osteoblasts cultured in the calcium titanate extracts prepared with polyethylene glycol, acetyl trim ethyl ammonium bromide and sodium citrate and without modifier was obviously improved under the concentration of 1.0 and 10 g/L. Absorbance value of the cells cultured in the above-mentioned four kinds of calcium titanate extracts 1.0 and 10 g/L） for 3, 6, 9 days exceeded the contrast （P 〈 0.05, P 〈 0.01）, as well as the alkaline phosphatase activity （P 〈 0.05, P 〈 0.01）. Calcium titanate prepared with polyethylene glycol, acetyl trim ethyl ammonium bromide and sodium citrate and without modifier has no cytotoxicity effect on osteoblasts, and can promote the proliferation and differentiation of osteoblasts.