| 高糖对肾小球系膜细胞Smurf2表达的影响 |
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| 引用本文： | 马红艳,肖斌,钟海花,顾峻林,徐玲,徐勇. 高糖对肾小球系膜细胞Smurf2表达的影响[J]. 重庆医学, 2010, 39(1) |
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| 作者姓名： | 马红艳 肖斌 钟海花 顾峻林 徐玲 徐勇 |
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| 作者单位： | 泸州医学院,附属医院内分泌科,四川泸州,646000;泸州医学院,生物化学教研室,四川泸州,646000;重庆市第七人民医院,400054 |
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| 基金项目： | 国家自然科学基金资助项目(No.30670980) |
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| 摘 要： | 目的观察不同浓度高糖刺激后大鼠肾小球系膜细胞胞内Smad泛素调节因子2(Smurf2)的表达,探讨泛素化降解在糖尿病肾病中的作用。方法将体外培养的大鼠肾系膜细胞分别设正常对照组(葡萄糖浓度5.6 mmol/L)、20 mmol/L高糖组、30 mmol/L高糖组、甘露醇组。分别用real ti me quantitative PCR法和细胞免疫荧光染色法及激光共聚焦显微镜检测各组细胞Smurf2的mRNA和蛋白的表达。结果(1)正常对照组系膜细胞Smurf2的mRNA和蛋白表达较弱。(2)高糖组Smurf2的mRNA和蛋白表达较正常对照组增强(P<0.05),呈浓度依赖性。结论高糖可诱导肾系膜细胞Smurf2表达增强。提示泛素-蛋白酶体途径可能参与了糖尿病肾病的病理进程。
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| 关 键 词： | 高糖 肾小球系膜细胞 Smad的泛素化调节因子 泛素-蛋白酶体途径 |
Effect of high glucose on expression of Smurf2 in cultured glomerular mesangial cells |
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| MA Hong-yan,XIAO Bin,ZHONG Hai-hua,et al.. Effect of high glucose on expression of Smurf2 in cultured glomerular mesangial cells[J]. Chongqing Medical Journal, 2010, 39(1) |
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| Authors: | MA Hong-yan XIAO Bin ZHONG Hai-hua et al. |
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| Affiliation: | MA Hong-yan1,XIAO Bin2,ZHONG Hai-hua1,et al. |
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| Abstract: | Objective To observe the expression of smad ubiquition regulatory factor2(Smurf2) in rat glomerular mesangial cells(GMC) stimulated by high glucose of different concentration,and to investigate the effect of the ubiquition in diabetic nephropathy.Methods Cultured rat GMC were divided into normal group(the concentration of glucose:5.6mmol/L),high glucose group(20mmol/L,30mmol/L respectively) and mannital group.The expression of Smurf2 mRNA of each group was measured by quantitative RT-PCR and protein was mea... |
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| Keywords: | high concentration glucose glomerular mesangial cell Smurf2 ubiquitin-proteasome pathway |
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