河北省淡色库蚊对常用杀虫剂的抗药性监测结果分析

目的监测河北省淡色库蚊对高效氯氰菊酯、氯菊酯、溴氰菊酯、仲丁威和敌敌畏5种常用杀虫剂的抗性动态。方法用WHO推荐的幼虫浸渍法,测定幼虫对常用杀虫剂的敏感性,获得半数致死浓度（LC50）及95%置信区间（95%CI）,比较其抗药性程度。结果 11个淡色库蚊野外种群对高效氯氰菊酯、氯菊酯、溴氰菊酯、仲丁威、敌敌畏的LC50分别为0.0008～0.1664、0.0159～0.2280、0.0006～0.0148、0.0800～0.6138和0.178～0.5000μg/ml。与1991-1994年的抗药性测定结果相比,河北省淡色库蚊对氯菊酯的抗性有增强趋势,对敌敌畏和溴氰菊酯的抗性有略微下降趋势。结论 11个淡色库蚊野外种群对5种杀虫剂均产生了不同程度的抗性,应加强抗药性监测,为科学合理使用杀虫剂提供指导。

Surveillance results of Culex pipiens pallens resistance to commonly used insecticides in Hebei province,China

Objective To investigate the resistance of Culex pipiens pallens to five commonly used insecticides in Hebei province, China. Methods The susceptibility of Cx. pipiens pallens larvae to commonly used insecticides was determined by WHO larval immersion method. Insecticide resistance was evaluated and compared based on median lethal concentration （LC_50） and 95% confidence interval （95% CI）. Results The LCs0 values of beta-cypermethrin, permethrin, dehamethrin, fenobucarb, and D DVP for 11 field populations of Cx. pipiens pallens were 0.0008-0.1664, 0.0159-0.2280, 0.0006-0.0148, 0.0800-0.6138, and 0.1783-0.5000 μg/ml, respectively. As compared with the previously reported results （1991-1994）, Cx. pipiens pallens showed increased resistance to permethrin and slightly decreased resistance to DDVP and dehamethrin. Conclusion The 11 field populations of Cx. pipiens pallens have developed different levels of insecticide resistance, The surveillance of insecticide resistance should be strengthened to provide guidance for the proper application of insecticides.