芦荟大黄素脂质体逆转人肺腺癌细胞A549/DDP对顺铂多药耐药的作用研究

目的：研究芦荟大黄素脂质体(aloe emodin liposomes，AE-L)在体外对耐药人肺腺癌细胞系A549/DDP对顺铂(DDP)的耐药性。方法：采用MTT检测方法得出AE-L的无毒细胞浓度、低毒细胞浓度以及空脂质体(empty liposomes,E-L)的无毒浓度；再以E-L无毒浓度做对照组，分别测出AE-L无毒细胞浓度组、低毒细胞浓度组耐药细胞逆转倍数；电感耦合等离子体质谱(ICP-MS)法测定各组细胞内顺铂浓度。结果：AE-L无细胞毒浓度组(2.0 mg·L^-1)使A549/DDP细胞对DDP的IC₅₀由16.81 mg·L^-1降至5.86 mg·L^-1；低细胞毒浓度(7.0 mg·L^-1)组的IC₅₀降至4.34 mg·L^-1；2组均明显提高DDP在A549/DDP细胞内的浓度。结论：AE-L能部分逆转A549/DDP细胞的多药耐药(MDR)，其机制与增加细胞内药物浓度有关。

Reversal effect of aloe emodin liposomes on cisplatin resistance lineA549/DDP human lung adenocarcinoma cells

Objective: To determine the reversal effect of aloe emodin liposomes (AE-L) on cisplatin resistance human lung adenocarcinoma cell line A549/DDP. Method: The un-cytotoxic and cytotoxic concentration of AE-L, and un-cytotoxic concentration of E-L were determined by MTT . The sensitivity of cisplatin were determined by MTT assay in above 3 groups. The intracellular concentration of cisplatin was detected by inductively coupled plasma mass spectrometry (ICP-MS). Result: The maximum non-toxic concentration group of AE-L (2.0 mg·L^-1) increased the sensitivity of cisplatin in A549/DDP, decreased IC₅₀ of cisplatin in A549/DDP from 16.81 mg·L^-1 to 5.86 mg·L^-1, and the hyp-cytotoxic concentration (7.0 mg·L^-1) group's IC₅₀ decreased to 4.34 mg·L^-1; AE-L groups significantly increased intracellular concentration of cisplatin in A549/DDP cells. Conclusion: The results showed that aloe emodin can reverse multidrug resistance (MDR) of A549/DDP cells and the mechanism might be associated with the increase of intracellular concentration of cisplatin.