小秋葵化学成分的分离与鉴定

目的研究小秋葵(Hibiscus trionumL.)的化学成分。方法采用反复硅胶柱色谱法、SephadexLH-20柱色谱法、重结晶法等进行分离纯化,通过理化常数测定和光谱分析鉴定其化学结构。结果从小秋葵中分离鉴定了8个化合物,即山柰酚(kaempferol,1)、槲皮素(quercetin,2)、芦丁(rutin,3)、山柰酚-3-O-(6-″O-反式对香豆酰基)-β-D-吡喃葡萄糖苷(kaem pfero l3-O-(6-″O-trans-p-cou-m aroy l)--βD-g lucopyranoside),4)、6-甲氧基-7-羟基香豆素(6-m ethoxy l-7-hydroxy lcoum arin,5)、6,7-二羟基香豆素(6,7-d ihydroxy lcoum arin,6)、β-谷甾醇(-βsitostero l,7)、胡萝卜苷(daucostero l,8)。结论化合物5、6为首次从木槿属植物中分离得到,化合物1~8为首次从小秋葵中分离得到。

Isolation and identification of chemical constituents from the whole plant of Hibiscus trionum L.

Objective To study the chemical constituents from the whole plant of Hibiscus trionum L. Methods The compounds were isolated by chromatography on silica gel, Sephadex LH-20 columns and the recrystallization methods. Chemical properties and NMR were employed for the structural identification. Results Eight compounds were isolated and identified as kaempferol (1), quercetin (2), rutin (3), kaempferol 3-O-(6-O-trans-p-coumaroyl)-β-D-glucopyranoside (4), 6-methoxyl-7-hydroxycoumarin (5), 6, 7-dihydroxycoumarin (6), β-sitosterol (7) and daucosterol (8), respectively. Conclusions Compounds 5, 6 are obtained from the Hibiscus genus for the first time and 1-8 are isolated from Hibiscus trionum L. for the first time.