淫羊藿及骨形态发生蛋白2与家兔的骨折愈合*

背景：淫羊藿是防治骨质疏松症的中药，但对骨折愈合的治疗尚缺乏依据。目的：观察中药淫羊藿对家兔骨折愈合的作用。方法：建立家兔单侧桡骨3 mm骨折动物模型，随机将其分为空白组、对照组和实验组。空白组予生理盐水灌胃加骨折局部注射生理盐水，实验组予中药淫羊藿熬制液灌胃加骨折局部注射生理盐水，对照组予生理盐水灌胃加骨折局部注射骨形态发生蛋白2。在术后2，4，8周，每组各取4只兔子通过X射线摄片、骨密度测定、生物力学测试(仅8周时)以及组织切片等评价骨折愈合的程度。 结果与结论：在各个时期段，X射线摄片显示实验组和对照组在骨痂的形成、改建及髓腔再通等均比空白组要好。实验组骨密度均优于空白组(P < 0.05)，实验组和对照组之间差异无显著性意义(P > 0.05)。实验组生物力学性能优于空白组(P < 0.05)，对照组数值介于两者之间。实验组和对照组在胶原纤维、软骨组织、骨小梁及骨基质的形成时期均早于空白组，实验组和对照组之间无显著性差异。提示淫羊藿能促进家兔骨折愈合。

Effect of epimedium and bone morphogenetic protein-2 on fracture healing in rabbits

BACKGROUND: Epimedium is a traditional Chinese medicine for osteoporosis prevention and treatment, but the treatment on fracture healing is still lack of basis. OBJECTIVE: To investigate the effect of epimedium on fracture healing in rabbits. METHODS: The models with 3mm fracture were established in half radius in rabbits, and were randomly divided into blank group, control group, and experimental group. The blank group was treated with normal saline by gastric perfusion and injected with normal saline in the part bone fracture. The experimental group was treated with epimedium by gastric perfusion and injected with normal saline. The control group was treated with normal saline by gastric perfusion and injected with bone morphogenetic protein-2 (BMP-2) in the part of bone fracture. At 2, 4, 8 weeks post-surgery, 4 rabbits were harvested to evaluate the degree of fracture healing according to X-ray radiography, bone density test, biomechanical test (only 8 weeks), and tissue section, etc. RESULTS AND CONCLUSION: X-ray radiograph showed that the experimental and control groups are better than the blank group in callus formation, remodeling, and recanalization of medullary cavity at each period. Bone densities in experimental group were prior to that in blank group (P < 0.05). There was no significant difference between the experimental group and control group (P > 0.05). Biomechanical properties of experimental group were better than that in the blank group (P < 0.05), the values of control group was between the other groups. The formation period of collagen fiber, cartilage tissue, bone trabecula, and bone matrix was earlier in the experimental and control groups compared with the blank group. There was no significant difference between the experimental and control groups. It is indicated that epimedium can promote fracture healing in rabbits.