Egb761对哮喘模型大鼠MMP-9及TIMP-1表达的影响和对气道重构的干预研究

目的:研究银杏叶提取物(Egb761)对哮喘大鼠气道上皮细胞基质金属蛋白酶-9(MMP-9)及其抑制剂-1(TIMP-1)表达的影响和对气道重构的干预作用。方法:40只大鼠随机均分为4组,即正常对照、哮喘模型、地塞米松及Egb761组,以吸入卵蛋白复制大鼠哮喘模型,用免疫组化技术结合计算机病理图像分析技术,测定各组大鼠气道形态学参数和气道上皮细胞MMP-9及TIMP-1表达的相对含量。结果:哮喘模型组大鼠气道壁的总厚度及内壁厚度和平滑肌层的厚度均显著高于正常对照、地塞米松和Egb761组(P<0.05);哮喘模型组MMP-9及TIMP-1表达量也显著高于上述3组(P<0.01);哮喘模型组MMP-9与TIMP-1的比例显著失衡。结论:少量多次的卵蛋白吸入可以导致大鼠气道重构的发生,MMP-9及TIMP-1在气道重构中发挥着重要作用,Egb761对哮喘模型大鼠的气道重构有干预作用。

Effects of Egb761 on the Expression of Matrix Metalloproteinase-9/Tissue Inhibitor of Metalloproteinase-1 in Asthmatic Model Rats and Its Intervention on Airway Remodeling

OBJECTIVE： To investigate the effects of extract of Ginkgo biloba （Egb761） on the expression of matrix metalloproteinase - 9（MMP - 9） and tissue inhibitor of metalloproteinase- I（TIMP - 1） and evaluate its intervention effect on airway remodeling in asthmatic rats model. METHODS： Rats were randomly assigned to normal control, asmatic group, Dexamethasone （DXM） group and Egb761 group. The asmatic model was established in rats. The changes of airway morphologic parameter and the relative content of MMP- 9 and TIMP - 1 in bronchial epithelium in these groups were determined using immunohistochemical technique and computer assisted image analysis system. RESULTS： The thickness of airway wall and airway smooth muscle was obviously thicker in the asthmatic group than any other group （P 〈 0.05） . The expression of MMP -9 and TIMP- 1 in the epithelial cells of bronchi was significantly higher in the asthmatic group than any other group （P〈0.01） and the ratio of MMP- 9/ TIMP- 1 was obviously disproportionate. CONCLUSION： Repeated exposure of small amount of OVA could induce airway remodeling. MMP - 9 and TIMP - 1 play an important role in airway remodeling. Egb761 exibited intervention effects on airway remodeling.