| 反相高效液相色谱法测定肠安胶囊中黄芩苷的含量 |
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| 引用本文： | 邵家德,周琴妹,陈晓斌,朱政中,徐燕,沈洪.反相高效液相色谱法测定肠安胶囊中黄芩苷的含量[J].中国药房,2005,16(5):378-379. |
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| 作者姓名： | 邵家德 周琴妹 陈晓斌 朱政中 徐燕 沈洪 |
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| 作者单位： | 江苏省中医院,南京市,210029 |
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| 摘 要： | 目的 :建立以反相高效液相色谱法测定肠安胶囊中黄芩苷含量的方法。方法 :色谱柱为C18,流动相为甲醇 -0 07 %磷酸溶液 (44∶56),流速为1ml/min ,检测波长为280nm ,柱温为室温。结果 :黄芩苷进样量在0 1054μg～1 0540μg范围内与峰面积呈良好的线性关系 (r=0 9996) ,平均加样回收率为97 44 % (RSD=2 92 % ,n=5)。结论 :本方法简便、准确、快速 ,可用于该制剂的含量测定。
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| 关 键 词： | 反相高效液相色谱法 肠安胶囊 黄芩苷 含量测定 |
| 文章编号： | 1001-0408(2005)05-0378-02 |
| 修稿时间： | 2004年6月29日 |
Content Determination of Baicalin in Chang'an Capsule by RP- HPLC |
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| SHAO Jiade,ZHOU Qinmei,Chen Xiaobin,ZHU Zhengzhong,XU Yan,SHEN Hong.Content Determination of Baicalin in Chang''''an Capsule by RP- HPLC[J].China Pharmacy,2005,16(5):378-379. |
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| Authors: | SHAO Jiade ZHOU Qinmei Chen Xiaobin ZHU Zhengzhong XU Yan SHEN Hong |
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| Abstract: | OBJECTIVE:To establish the method for determination of baicalin in Chang’an capsule by RP-HPLC. METHODS:HPLC was performed on C 18 column with methanol-0.07%phosphoric acid solution(44∶56)as mobile phase at a flow rate of lml/min and the temperature of column kept at room temperature.The UV detection wavelength was280nm.RESULTS:The linear range of baicalin was sample size0.1054μg～1.0540μg(r=0.9996),the average recovery was97.44%(RSD=2.92%,n=5).CONCLUSION:The method was simple,accurate and fast,and can be used for the determination of baicalin. |
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| Keywords: | RP-HPLC Chang’an capsule Baicalin Determination of content |
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