高脂饮食加低剂量链脲霉素建立小鼠2型糖尿病模型

目的高脂饮食加低剂量链脲霉素(Streptozotocin,STZ)建立小鼠2型糖尿病模型。方法5周的雄性C57BL/6J小鼠,随机分为正常饲料组、正常饲料加STZ组、高脂饲料组和高脂饲料加STZ组。相应饲料喂养5周后,按照100 mg/Kg的剂量腹腔注射STZ,然后继续喂养4周。在第5周和第9周末测定小鼠的体重、收缩压、血糖、血胰岛素、血甘油三脂和胆固醇水平。结果STZ注射前各组小鼠的体重、血压、血糖、血胰岛素、血脂和血甘油三脂无明显差异(P0.05)。STZ注射后4周时,高脂饲料加STZ组小鼠的体重、血糖、血胰岛素、血压和血脂水平明显升高(P0.05);而其他三组的这些指标无明显改变或仅部分升高。结论高脂饮食加低剂量链脲霉素可建立小鼠2型糖尿病模型,该模型具有人2型糖尿病的主要表型特征和相似的发病过程。

Model Establishment of Type 2 Diabetes Mellitus Induced by High Fat Diet with Low Dose Streptozotocin in C57BL/6J Mice

Objective To develop a mice model of type 2 diabetes mellitus(DM) by high fat diet with low dose streptozotocin(STZ).Methods\ Male C57BL/6J mice at 5 weeks of age were randomly divide into conventional diet(n=8),conventional diet with STZ(n=8),high fat diet(n=8) and high fat diet with STZ group(n=8).After the exposure to conventional or high fat diet for 5 weeks,mice were injected intraperitoneally with 100 mg/kg of either STZ or vehicle solution during fasting state,and kept on the same diet for the next 4...