| 上皮性肿瘤标记物桥粒的提取 |
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| 引用本文： | 杨举伦,毛敏,刘开凤,杨光华,孙芝琳,林晰. 上皮性肿瘤标记物桥粒的提取[J]. 四川大学学报(医学版), 1991, 0(3) |
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| 作者姓名： | 杨举伦 毛敏 刘开凤 杨光华 孙芝琳 林晰 |
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| 作者单位： | 华西医科大学病理学教研室(杨举伦，毛敏，刘开凤，杨光华)，华西医科大学生物化学教研室(孙芝琳)，华西医科大学生物化学教研室(林晰) |
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| 摘 要： | 本文报道一种简单易行的桥粒提取方法。取新鲜牛鼻表皮棘层,以pH2.3含0.5mmol/L苯甲基磺酰氟、0.1mol/L枸椽酸-枸椽酸钠缓冲液浸泡,匀浆,经蔗糖密度梯度离心,得到纯桥粒。电镜证实桥粒结构保存完好,附在桥粒斑上的中丝部分消失。209牛鼻表皮棘层可提取桥粒约100mg。
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| 关 键 词： | 肿瘤 标记物 桥粒 提取 |
Isolation of Desmosomes:A Marker for Epithelial Tumors |
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| Yang Julun,Mao Min,Liu Kaifeng,Yang Guanghua,Sun Zhilin,Lin Xi. Isolation of Desmosomes:A Marker for Epithelial Tumors[J]. Journal of Sichuan University. Medical science edition, 1991, 0(3) |
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| Authors: | Yang Julun Mao Min Liu Kaifeng Yang Guanghua Sun Zhilin Lin Xi |
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| Affiliation: | Yang Julun;Mao Min;Liu Kaifeng;Yang Guanghua;Sun Zhilin;Lin Xi Department of Pathology Department of Biochemistry |
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| Abstract: | A simple method is repor-ted for the isolation of desmosomes. Thefresh noncornified layers of cow nose epi-dermis were immersed in 0.1 mol/L citricacid-sodium citrate buffer (pH 2.3, con-taining 0.5mmol/L PMSF),and then trea-ted with homogenization and discontinuoussucrose density gradient centrifugation.Desmosomes were located at the 50-56%sucrose interface. Electron microscopy re-vealed that the characteristic desmosomestructure was well preserved.and that afew intermediate filament bundles attachedto desmosome plaque were removed bythe solubilizing action of the buffer. Ap-proximate 100 mg desmosomes(dry weight)were got from 20g wet noncornified layersof epidermic tissue. |
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| Keywords: | Classification Tumor Marker Desmosome Isolation |
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