高效液相色谱法测定水红花子中芦丁与槲皮苷的含量

 目的用高效液相色谱法测定水红花子中芦丁与槲皮苷的含量。方法芦丁的含量采用Shimadzu C₁₈柱(4.6mm×150mm,5μm),流动相为甲醇-0.05%H₃PO₄溶液(42:58),流速1.0mL·min^-1,检测波长254nm,柱温25℃,用外标法定量;采用Shimadzu C₁₈柱(4.6mm×150mm,5μm),流动相为甲醇-0.2%H₃PO₄溶液(45:55),流速1.0mL·min^-1,检测波长350nm,柱温室温,用外标法定量,测定槲皮苷的含量。结果芦丁的线性范围0.053～0.53μg,r=0.9999;槲皮苷的线性范围0.40～2.00μg,r=0.9996。结论该方法简便,快速,线性关系良好。

Determination of rufin and quercitrin in Polygonum orientale by HPLC

OBJECTIVE To determine the contents of rutin and quercitrin in polygonum orientale by HPLC. METHODS The analysis of rutin was perforated on a Shimadzu C₁₈ column (4.6 mm×150 mm, 5 μm). The mobile phase was MeOH-0.05%H₃PO₄(42: 58) with a flow rate of 1.0 mL·min^-1 at 254 nm. Quercitrin was separated on a Shimadzu C₁₈ column(4.6 min×150 mm,5 μm).The mobile phase was MeOH-0.2% H₃PO_{4(45: 55) with a flow-rote of 1.0 mL·min^-1 at 350 nm. Both seperation were performed at room temperature. RESULTS The method was simple and had a good linear relationship. The linear range of rutin was 0.053～0.53 μg with a good correlation (r) of 0.9999. The recovery was 96.30%, RSD was 1.22%. The linear range of quercitrin was 0.40～2.00 μg with a good correlation (r) of 0.9996. The recovery was 97.80%, RSD was 1.43 %. CONCLUSION The method is rapid, accurate and can be used to determine the contents of rutin and quercitrin in Polygonum orientale.}