Influence of the physical and chemical properties of magnetic nanoparticles on their performance in a chemiluminescence immunoassay |
| |
| Authors: | Xiaoyan Dai Hong Xu Yongguang Li Hongchen Gu Meng Wei |
| |
| Affiliation: | 1. Division of Cardiology, Shanghai Jiao Tong University Affiliated Sixth People''s Hospital, Shanghai, 200233, PR China;2. Nano Biomedical Research Center, School of Biomedical Engineering, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, PR China |
| |
| Abstract: | ObjectivesMagnetic nanoparticles (MNPs) are important carriers in immunoassays. In this study, we investigated the influence of the physical and chemical properties of MNPs on their performance in a detection process.Design and methodsA comparative study of the properties of two MNPs with sizes of 200 nm and 1 μm (MNP-200 nm and MNP-1 μm, respectively) was conducted using the following four aspects: nonspecific adsorption to proteins (IgG was used as a model protein), influence of magnetic nanoparticles on the chemiluminescence signal, response speed to an external magnetic field, and intensity of the detection signal.ResultsMNP-1 μm exhibited lower nonspecific adsorption to IgG in serum, a weaker interference with the chemiluminescence signal, and a higher response speed to the external magnetic field than the same amount of MNP-200 nm. An automated chemiluminescence immunoassay system based on MNP-1 μm was also established.ConclusionsMNP-1 μm acts as an excellent carrier in an automated chemiluminescence immunoassay system for the analysis of serum samples from clinical patients. |
| |
| Keywords: | Magnetic nanoparticle Physicochemical property Chemiluminescence immunoassay Detection performance Myoglobin |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
