两种十大功劳叶中生物碱成分的分析与比较

目的建立长柱十大功劳叶和阔叶十大功劳叶中药根碱、巴马汀、小檗碱的含量测定方法，比较两者的生物碱成分含量差异。方法采用反相高效液相色谱外标法同时测定。色谱柱为大连依利特Sino Chrom ODS—BP柱（250mm×4．6mm，5μm），流动相为乙腈-0．2％磷酸（23：77），流速为1．0mL／min，检测波长为265nm，进样量为10μL。结果上述色谱条件下，盐酸药根碱、盐酸巴马汀、盐酸小檗碱可以较好地分离，线性范围分别为0．001444—0．07076μg（r=0．9999），0．0005152～0．02524μg（r=0．9996），0．0009240-0．04528μg（r=0．9998），平均回收率分别为98．74％，99．37％，99．89％，RSD分别为2．16％，1．62％，2．14％（n：9）。结论该测定方法简单，快速，专属性、重现性好，结果准确可靠。

Alkaloids Analysis and Comparison of Two Kinds of Mahonia Bealei Leaves

Objective To establish a HPLC method to determine the content of jatrorrhizine, palmatine and berberine in the leaves of Mahonia bealei（Fort. ） Carr. and Mahonia duclouxiana Gagnep. and to compare the difference in the alkaloids contents between the two kinds of leaves. Methods The HPLC condition was as follows： Dalian Elite Sino Chorm ODS-BP column （250 mm ×4. 6 mm, 5 μm）, the mobile phase consisted of acetonitrile-0.2%H3PO4（23 ： 77）, the flow rate at 1.0 mL/min and the detection wavelength at 265 nm. Results The calibrated linear curves of jatrorrhizine hydrochloride, palmatine hydrochloride and berberine hydrochloride concentrations were within the range of 0. 001 444 - 0. 070 76, O. 000 515 2 - 0. 025 24 and 0. 000 924 0 - 0. 045 28 μg, and the correlation coefficients（r） were 0. 999 9, 0. 999 6, 0. 999 8, respectively. The recovery rates were 98.74% （ RSD = 2. 16% ）, 99.37% （ RSD = 1.62% ）, 99.89% （ RSD = 2. 14% ）, respectively. Conclusion The HPLC method is simple, rapid and good in specificity and reproducibility with reliable and accurate results. The results prove that the leaves of Mahonia bealei（Fort. ） Cart. and Mahonia duclouxiana Gagnep. have the similar chemical constituents.