肿瘤化疗药物对人LAK细胞活性的影响

用四氮唑（ＭＴＴ）法分别检测顺氨氯铂（ＰＤＤ），丝裂霉素－Ｃ（ＭＭＣ）、阿霉素（ＡＭＤ）、氟尿嘧啶（５－Ｆｕ），阿糖胞苷（Ａｒａ－ｃ），长春新碱（ＶＣＲ）对ＩＬ－２诱导人外周血ＬＡＫ细胞活性的影响，及其与ＬＡＫ细胞对胃癌细胞的相加杀伤作用。①ＭＭＣ、ＡＤＭ、５－Ｆｕ、ＶＣＲ对ＩＬ－２诱导ＬＡＫ细胞活性稍有下降，Ａｒａ－Ｃ能抑制ＩＬ－２诱导ＬＡＫ细胞活性，ＰＤＤ对ＩＬ－２诱导ＬＡＫ细胞活性明显提高。

EFFECTS OF CHEMOTHERAPEUTIC DRUGS ON THE CYTOADTIVITY OF LYMPHOKINE ACTIVATED KILLER CELLS IN VITRO

he effects of PDD , MMC,5-Fu , Ara-C and VCR on the activity of LAK cells in the periph-eral blood activated by IL-2 were respectively and their additive killing action together with LAK cellon the gastric carcinoma cells were tested with MTT method. The results were as follows: (l) Theeffects of four kinds of chemotherapeutic drtigs,such as MMC,ADM,5-Fu and VCR,on the activityof LAK cell activated by IL-2 were slightly decreased, but there was no significant difference as com-pared with the contrast group(P>0.05). Ara-C could inhibit the activity of LAK cell activated byIL-2(P<0.05, as compared with the contrast group).PDD could be markedly elevated(P<0.01,as compared with the contrast group).(2) Five kinds of chemotherapeutic drugs, such as PDD,ADM, 5-Fu,Ara-C and VCR had cytotoxic effects added to LAK cell on the gastric carcinoma cells(P<0.05),but MMC did not have such an additive effects. It suggested that chemotherapeutic drugssensitive to tumor cells and synergic with LAK cell activities could be selected to use with LAK ascombination therapy.