| 两种构建大鼠膀胱癌模型方法的比较研究 |
| |
| 引用本文： | 李云,许传亮,李志华,杨庆,顾正勤,王林辉,孙颖浩. 两种构建大鼠膀胱癌模型方法的比较研究[J]. 临床泌尿外科杂志, 2008, 23(10) |
| |
| 作者姓名： | 李云 许传亮 李志华 杨庆 顾正勤 王林辉 孙颖浩 |
| |
| 作者单位： | 第二军医大学附属长海医院泌尿外科,上海,200433;上海市市北医院泌尿外科;第二军医大学附属长海医院泌尿外科,上海,200433;上海市市北医院泌尿外科 |
| |
| 基金项目： | 上海市闸北区科委医学科学研究基金，上海市卫生局资助项目 |
| |
| 摘 要： | 目的:通过N-正丁基-N-(4-羟基-丁基)亚硝胺[N-butly-N-(4-hydroxybutyl)nitrosamine,BBN](BBN)喂饲和MNU膀胱灌注方法构建大鼠膀胱癌模型,比较两种实验方法的优缺点.方法:BBN诱癌组大鼠使用0.05% BBN溶液连续喂养六周.后改用2%枸橼酸钠溶液作后续喂养22周,第28周为诱导的终点.MNU诱癌组大鼠行MNU膀胱灌注,每两周灌药1次,每次2 mg/只.共4次.第10周为诱导的终点.比较两组大鼠的成瘤率和死亡率.结果:BBN诱癌组30只大鼠喂养28周后存活16只,11只成瘤,死亡率46.7%,存活大鼠成瘤率68.8%;MNU诱癌组30只大鼠第10周存活28只,25只成瘤,死亡率6.7%,存活大鼠成瘤率89.3%.结论:利用MNU行大鼠膀胱灌注的方法构建大鼠膀胱癌模型,具有成瘤时间短、成瘤率高、大鼠死亡率低等特点,优于BBN喂饲大鼠成瘤的方法.
|
| 关 键 词： | 膀胱肿瘤 化学致癌剂 N-正丁基-N-(4-羟基-丁基)亚硝胺 N-甲基亚硝基脲 动物模型 |
The comparative study in the construction of experimental animal model of bladder tumor in rat induced by BBN and MNU |
| |
| LI Yun,XU Chuanliang,LI Zhihua,YANG Qing,GU Zhengqin,WANG Linhui,SUN Yinghao. The comparative study in the construction of experimental animal model of bladder tumor in rat induced by BBN and MNU[J]. Journal of Clinical Urology, 2008, 23(10) |
| |
| Authors: | LI Yun XU Chuanliang LI Zhihua YANG Qing GU Zhengqin WANG Linhui SUN Yinghao |
| |
| Affiliation: | 1Department of Urology;the Changhai Hospital;Second Military Medical University;Shanghai;200433;China;2Department of Urology;Shibei Hospital;Shanghai |
| |
| Abstract: | Objective:To compare the advantages and disadvantages in the construction of experimental animal model of bladder tumor in rat induced by BBN and MNU respectively.Methods:A total of 30 female SD rats weighing about 180~220 gm were given drinking water contain 0.05% BBN for 6 weeks continuously, then fed 2% natrium citricum solution for 22 weeks, 28 weeks later all rats were killed. Other 30 female SD rats were infused intravesically with 2 mg MNU 4 times, at 2 weeks interval, 10 weeks later all rats were ki... |
| |
| Keywords: | |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
