HIV/HCV共感染对HIV一1生物学特性影响的研究

目的研究有偿献血人群HIV／HCV共感染对HIV-1基因离散率、病毒载量、CD4 T淋巴细胞等生物学特性影响。方法扩增HIV-1 env基因C2～V3区,Clustal W和Phylip软件计算基因离散率,NASBA方法检测HIV-1病毒载量,采用Coulter公司的Epics XL流式细胞计数仪检测CD4 细胞数,用SPSS软件t检验分析HIV／HCV共感染对HIV-1生物学特性的影响。结果HCV共感染与非共感染人群的HIV-1平均基因离散率为7．95％、15．73％(P<0．001),平均病毒载量为4．61、4．45(P=0．522),平均CD4 T淋巴细胞数为308、251(P=0．161)。结论该有偿献血人群中HIV／HCV共感染对HIV-1基因离散率有影响,而对病毒载量、CD4 T淋巴细胞无影响。

Study on the influence of biological characteristics to HIV/HCV co-infection among HIV infected iIlegal blood donors in China

Objective To study the influence of biological characteristics on HCV/HIV co-infection, including HIV-1 sequence distance, HIV-1 viral load and CD4 count. Methods HIV-1 sequence distance was calculated by Clustal W and Phylip software while HIV-1 viral load being tested by NASBA and CD4 count was tested using Epics XL of Coulter. Significance was determined by t-test using SPSS 12.0. Results The mean HIV-1 genetic distances were 7.95% and 15. 73% (P< 0.001) between those with HCV co-infection and those without. Their mean HIV-1 viral loads were 4.61 and 4.45(P = 0. 522) and their mean CD4 T counts were 308 and 251(P = 0.161), respectively. Conclusion Data showed that in the study group, the HIV/HCV co-infection had an influence on the HIV sequence distance, but did not have major impact on HIV-1 viral load and their mean CD4 T count.