| IQGAP1对甲醛致小鼠气道上皮损伤修复作用 |
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| 引用本文： | 葛荣,朱晓蔚,李娜萍,吴人亮. IQGAP1对甲醛致小鼠气道上皮损伤修复作用[J]. 中国公共卫生, 2008, 24(11) |
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| 作者姓名： | 葛荣 朱晓蔚 李娜萍 吴人亮 |
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| 作者单位： | 华中科技大学同济医学院同济医院病理研究所,卫生部呼吸系统疾病重点实验室,武汉,430030 |
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| 摘 要： | 目的 探讨包含IQ序列的三磷酸鸟苷酶(GTP)激活蛋白1(IQGAPl)和细胞分裂周期42蛋白(Cdc42)在气道上皮细胞损伤修复不同阶段的作用.方法 选用健康昆明种小鼠24只,5mg.m3气态甲醛,染毒4h.d,分别染毒3.7和60d后处死,采用免疫组织化学染色、蛋白印迹(Western blot)检测和免疫荧光共聚焦成像方法,观察IQ-GAP1和Cdc42蛋白在染毒小鼠气道上皮细胞损伤修复过程中的表达和定位变化.结果 IQGAP1在染毒3和7d时表达明显增强(P＜0.01),60d时恢复至对照组水平;Cdc42蛋白在染毒3d时表达明显减弱(P＜0.01),7d时表达有所恢复.对照组中IQGAP1主要定位于气道上皮细胞的胞膜处,而Cdc42蛋白弥散分布于气道上皮细胞(AECs)胞质中;3d和7d时共同定位于细胞极化前缘;60d时其定位与对照组相似.结论 Cdc42蛋白和IQGAP1在气道上皮细胞损伤修复过程中的表达及定位呈动态变化,提示其在这一过程中发挥着重要作用.
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| 关 键 词： | IQ序列三磷酸鸟苷酶激活蛋白 细胞分裂周期42蛋白 气态甲醛 气道上皮 损伤修复 |
Roles of Cdc42 protein and IQGAP1 in the repair of airway epithelial cells after injured by gaseous formaldehyde in mice |
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| GE Rong,ZHU Xiao-wei,LI Na-ping,et al.. Roles of Cdc42 protein and IQGAP1 in the repair of airway epithelial cells after injured by gaseous formaldehyde in mice[J]. Chinese Journal of Public Health, 2008, 24(11) |
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| Authors: | GE Rong ZHU Xiao-wei LI Na-ping et al. |
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| Affiliation: | GE Rong,ZHU Xiao-wei,LI Na-ping,et al.Institute of Pathology,Tongji Hospital.Tongji Medical College,Huazhong University of Science , Technology(Wuhan 430030,China) |
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| Abstract: | Objective To investigate the roles of IQ motif containing GTPase activating protein 1(IQGAP1) and cell division cycle 42 protein(Cdc42) in the repair of the injured airway epithelial cells(AECs) at different stages.Methods Twenty-four Kunming mice were randomly divided into four groups as following:3,7,60-day formaldehyde(FA) inhalation group and the control group.The mice were exposed to gaseous FA of 5 mg/m3 for 4h/day for 3,7,and 60 days.The expressions and locaiizations of IQGAP1 and Cdc42 protein in th... |
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| Keywords: | IQGAP1 Cdc42 protein gaseous formaidehyde airway epithelial cell repair |
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