小鼠病毒性心肌炎与细胞凋亡及氧自由基的关系

采用电镜技术及原位末端标记法（ＴＵＮＥＬ）对柯萨奇病毒Ｂ３（ＣＶＢ３）心肌炎小鼠不同时期心肌组织中细胞凋亡进行检测，并采用硫代巴比妥酸法检测外周血丙二醛（ＭＤＡ）。ＣＶＢ３感染１００只雄性Ｂａｌｂ／ｃ小鼠，ＶＭＣ发病率为８６％。各期病鼠心肌中均见到凋亡细胞，包括心肌细胞、血管内皮细胞及间质细胞。ＴＵＮＥＬ法检出率为７８．９４％，中、重度ＶＭＣ小鼠中阳性检出率明显高于轻度ＶＭＣ小鼠（Ｐ〈０．０５）。

The Relationship of Viral Myocarditis,Apoptosis and Oxygen Free Radical

Electronic microscopic and terminal trans ferasemediated dUPT biotin nick end labeling(TUNEL)assays quantified apoptosis in myocardium Malon dialdehyde(MDA)in serum were detected One hundred Balb/c mice were inoculated with CVB 3 The incidence of VMC was 86% Apoptotic cells detected in myocardium from CVB 3 injected mice included myocytes,endothelial cells and infiltrating cells in myocardium TUNEL positive myocytes increased significantly from 7 to 14 days p i then reduced from 21 to 28 days p i(P<0 05) The positive rate of TUNEL positive mice were more higher in mice exhibited moderate to severeer histopathology than that in mice exhibited mild histopathology(P<0 05) In experimental group,the level of MDA rised up significantly from 7 days p i and reached its peak at 14 days p i ,recovered from 28 days p i The level of MDA in serum from CVB 3 injected mice correlated with the degree of myocardial lesions significantly(P<0 05) Both of Apoptosis and MDA which can induce apoptosis significantly during 7 to 14 days p i It demonstrates that apoptosis might play an important role in the development of VMC Oxygen free radicals might be one of proapoptosis factors