紫杉醇微乳抗肿瘤作用的研究

目的采用薏米仁油研制紫杉醇微乳,评价其抗肿瘤作用。方法采用聚乙二醇-二硬脂酰基磷脂酰乙醇胺（PEG-DSPE）和薏米仁油,制备紫杉醇微乳,并测定其Zeta电位和粒度分布。评价紫杉醇微乳对小鼠的急性毒性及其抗肿瘤作用。结果紫杉醇微乳粒径为112.6±2.5nm,Zeta电位为-32.4±3.5mV。在本实验条件下,紫杉醇微乳显著降低了紫杉醇的急性毒性,紫杉醇和薏米仁油具有显著的协同抗肿瘤作用（P〈0.01）。结论我们制备的紫杉醇微乳有可能成为一种较好的抗肿瘤药物。

Anticancer effect of paclitaxel carried with coix seed oil microemulsions

Objective To prepare coix seed oil microemulsion to carry paclitaxel and evaluate its acute toxicity and anticaneer effect. Methods The paclitaxel microemulsion was a semi-transparent solution containing paclitaxel, coxi seed oil and PEG-DSPE （polylene glycol derivative of distearoyl phosphatidyl ethanolamine, mean molecular weight of PEG： 2000）. The Zeta potential and particle size distribution were determined using a laser light scattering instrument. The acute toxicity of the pacli- taxel microemulsion was studied using mice and its anticancer effect was evaluated using Lewis lung and M5076 tumor models. Results The average diameter of the paclitaxel microemulsion was 112.6±2.5nm and its Zeta potential was -32.4±3.5mV while its paclitaxel concentration was 0.29mg/mL. Compared with paclitaxe and coxi seed oill, the paclitaxel microemulsions showed lower acute toxicity and synergysitic anticancer effect. Conclusion The paclitaxel microemulsion may become a better anti- cancer drug than paclitaxel and coxi seed oil.