FR/CL体外干预人胃癌细胞株SGC-7901及人肺癌细胞株A549生长

目的 研究金荞麦提取物FR联合刺梨提取物CL对人胃癌细胞株SGC-7901及人肺癌细胞株A549增殖及凋亡的影响.方法 MTT法检测FR、CL对SGC-7901、A549细胞的增殖抑制率;流式细胞术分析早期凋亡细胞比; RT-PCR、Western blot分析Ki-67及Bax、Bcl-2表达的变化.结果 FR、CL单药均可明显抑制SGC-7901、A549细胞的生长,呈剂量依赖性;两药IC_(30) 浓度联合呈增效效应; IC_(30) 浓度FR、CL单药和1/2 IC_(30) (FR)+1/2 IC_(30) (CL)联合对SGC-7901细胞的抑制率分别为(33.89±0.22)%、(30.86±1.17)%和(44.25±0.50)%,对A549细胞的抑制率分别为(30.91±1.72)%、(29.07±1.41)%和(43.62±2.15)%.流式细胞术结果表明,对照组、FR组、CL组、联合组细胞早期凋亡比例依次增加,两单药组及联合组与对照组之间、联合组分别与两单药组之间的差异均具统计学意义(P<0.05);与单药组比较,联合组Ki-67、Bcl-2 mRNA/蛋白表达率有显著降低,而Bax mRNA/蛋白表达显著增高.结论 FR、CL均可抑制人胃癌细胞株SGC-7901细胞、人肺癌细胞株A549的增殖和诱导其凋亡,且联合应用存在增效效应.

Extracts of Fagopyrum cymosum and Rosa roxburghii Tratt inhibit proliferation and induce apoptosis of human gastric and pulmonary carcinoma cells

Objective To investigate the effects of extract of Fagopyrum cymosum (FR) and Rosa roxburghii Tratt (CL) on the human gastric carcinoma SGC-7901 and pulmonary carcinoma A549 cell line. Methods Human gastric carcinoma SGC-7901 cells and pulmonary carcinoma A549 cells were exposed to FR and/or CL in vitro. The inhibitory effects were evaluated by MTT assay. Cell apoptosis was detected by flow cytometry. The expressions of Ki-67,Bax and Bcl-2 in mRNA and protein level were quantified by RT-PCR and Western blot. Results The growth inhibition of SGC-7901 and A549 was dose-dependent after exposure to the FR or CL alone. The inhibitory rates of FR at IC_(30) ,CL at IC_(30) and FR plus CL at 1/2 IC_(30) were (33.89±0.22)%,(30.86±1.17)% and(44.25±0.50)% for SGC-7901; and(30.91±1.72)%,(29.07±1.41)% and(43.62±2.15)% for A549,respectively. The cells apoptotic rates increased in order of control,FR,CL and FR plus CL group (P<0.05). Compared with the FR or CL,the expressions of Ki-67 and Bcl-2 mRNA/protein in FR plus CL group were decreased but the expression of Bax mRNA/protein was increased,both for SGC-7901 and A549 cells. Conclusion Both FR and CL have inhibitory and apoptosis-inducing effects on the human gastric carcinoma SGC-7901 and pulmonary carcinoma A549 cell line,and the combination of FR and CL shows a synergistic effect.