结直肠癌中P53和Kras基因突变与血管内皮生长因子表达的关系

目的探讨结直肠癌中P53和K-ras基因突变与血管内皮生长因子(VEGF)的关系。方法采用RT-PCR技术和聚合酶链式反应-单链构像多态性分析(PCR-SSCP)方法,对46例结直肠癌的新鲜癌组织进行P53、K-ras基因突变率和VEGF表达率的检测。结果46例结直肠癌中VEGF表达阳性率为52.2%(24/46),P53基因突变率为63.0%(29/46),K-ras基因突变率为43.4%(20/46)。P53基因突变的29例中VEGF表达阳性20例,占68.9%,表达阴性9例,占31.0%,P<0.05。K-ras基因突变的20例中VEGF表达阳性14例,占70.0%,表达阴性6例,占30%,P<0.05。结论结直肠癌组织中P53基因突变和K-ras基因突变与VEGF表达呈正相关,此点为临床基因治疗肿瘤提供了重要理论依据。

Relationship between mutant genes of P53 and K ras and expression of vascular endothelial growth factor in colorectal cancer

Objective To study the relationship between mutant genes of P53 and K ras and expression of vascular endothelial growth factor(VEGF) in colorectal cancer.Method RT PCR and PCR SSCP were used to detect P53, K ras gene and VEGF in primary foci of 46 cases with colorectal cancer.Results The positive expression rates of VEGF, P53 and K ras mutation genes were 52.2%(24/46), 63.0%(29/46) and 43.4%(20/46) respectively. Positive expression rate of VEGF was 68.9%(20/29) in 29 cases with positive P53,and 70%(14/20) in 20 cases with positive K ras. Both were significantly different as compared to the negative expression rates of VEGF (P< 0 05).Conclusion Mutant genes of P53 and K ras are correlated with VEGF significantly.