| 细胞外液酸碱度对主动脉平滑肌细胞电生理的影响 |
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| 引用本文： | 黄民,刘威,张春晓,曹晓杰. 细胞外液酸碱度对主动脉平滑肌细胞电生理的影响[J]. 吉林大学学报(医学版), 2003, 29(5): 551-554 |
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| 作者姓名： | 黄民 刘威 张春晓 曹晓杰 |
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| 作者单位： | 吉林大学基础医学院生理学教研室,吉林,长春,130021;吉林省中医中药研究院 |
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| 基金项目： | 国家自然科学基金;39800226; |
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| 摘 要： | 目的 :探讨细胞外液酸碱度 ( p H0 )的改变对主动脉平滑肌细胞电生理特性的影响。方法 :通过改变培养的乳鼠主动脉平滑肌细胞外液的酸碱度 ,应用全细胞膜片钳技术记录细胞膜的变化和钾离子通道活动的情况。结果 :p H0 降低时引起细胞膜超极化 ,外向钾电流增大 ;反之 ,p H0升高时引起细胞膜去极化 ,外向钾电流减小。这种变化可以被 TEA阻断 ,而对 4- AP不敏感。结论 :p H0 改变引起主动脉平滑肌细胞膜电位及外向电流幅值变化 ,其机制可能与钙激活性钾通道的开放几率有关
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| 关 键 词： | 细胞外隙 酸碱平衡 主动脉/细胞学 肌 平滑 血管/生理学 膜片钳术 电生理学 |
| 文章编号： | 1671-587X(2003)05-0551-04 |
| 修稿时间： | 2002-04-29 |
Effects of pH0 on Electrophysiological Activities of Aortic Smooth Muscle Cells |
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| HUAN Min ,LIU Wei ,ZHANG Chun xiao ,CAO Xiao jie. Effects of pH0 on Electrophysiological Activities of Aortic Smooth Muscle Cells[J]. Journal of Jilin University: Med Ed, 2003, 29(5): 551-554 |
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| Authors: | HUAN Min LIU Wei ZHANG Chun xiao CAO Xiao jie |
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| Affiliation: | HUAN Min 1,LIU Wei 2,ZHANG Chun xiao 1,CAO Xiao jie 1* |
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| Abstract: | Objective: To study on the pH 0 effects of changes on membrane potential and K + channels activity in aortic smooth muscle cells. Methods: The extracellular fluid pH 0 of cultivated aortic smooth cells was changed, then the membrane potential and K + currents were recorded with whole cell patch clamp technique. Results: The membrane potential hyperpolarized and outward K + currents increased with the decreasing pH 0. On the contrary, the membrane potential depolarized and outward K + currents decreased with the elevating pH 0 and the changes could be blocked by TEA but not by 4 AP. Conclusion:The changes of membrane potential and K + channels in aortic smooth muscle cells induced by pH 0 may be connected with the open activity of the Ca activated K + channels. [ |
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| Keywords: | Extracellular space Acid base balance Aorta/cytology Muscle smooth blood vessel/physiology Patch clamp technique Electrophysiology |
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