木香超临界水提液对豚鼠离体肠和小鼠肠推动的作用研究

目的观察木香超临界后水提液对小鼠小肠推进运动和豚鼠离体回肠平滑肌收缩的影响。方法以正常、新斯的明负荷和肾上腺素负荷为研究模型,采用碳末推动法和离体肠实验方法。结果木香Ⅰ组、木香Ⅱ组和木香Ⅲ组碳末推进率均高于正常对照组,差异有统计学意义（P〈0.05和P〈0.01）。肾上腺素组碳末推进率明显低于正常对照组,差异有统计学意义（P〈0.01）;木香Ⅰ组、木香Ⅱ组和木香Ⅲ组碳末推进率明显高于肾上腺素组,差异有统计学意义（P〈0.01）。新斯的明组碳末推进率明显高于正常对照组,差异有统计学意义（P〈0.01）;木香Ⅰ组、木香Ⅱ组和木香Ⅲ组碳末推进率均低于新斯的明组,差异有统计学意义（P〈0.05和P〈0.01）。给药后豚鼠自发收缩活动频率和振幅均高于给药前,差异有统计学意义（P〈0.05和P〈0·01）,且振幅随着木香超临界水提液剂量的增加而增大。结论木香超临界水提液有促进小鼠小肠运动的作用,对肾上腺素所致小鼠小肠推进抑制有显著的促进作用,对新斯的明所致的小鼠小肠推进亢进有拮抗作用,对豚鼠离体回肠自发活动有兴奋作用。

Effect study of the supercritical aqueous extract of radices saussurease on propulsive movement of guinea-pig intestine in vitro and mouse intestine

Objective To oberve the effect of the supercritical aqueous extract of radices saussurease on the propulsive movement of mouse small intestine and the contraction of guinea-pig ileum smooth muscle in vitro.Methods The normal mice,neostigmine load mice and adrenaline load mice were regarded as studied model,the method of propelling carbon powder and isolated intestine test were used.Results The propellant rates of carbon powder of radices saussurease group Ⅰ,radices saussureuse group Ⅱ and radices saussureuse group Ⅲ were higher than those of the normal control group,the differences were statistically significant (P<0.05 and P<0.01).The propellant rate of carbon powder of the adrenaline group was lower than that of the normal control group obviously,the difference was statistically significant(P<0.01);the propellant rates of carbon powder of the radices saussurease group Ⅰ,radices saussurease group Ⅱ and radices saussurease group Ⅲ were higher than those of the adrenaline group,the differences were statistically significant(P<0.01).The propellant rate of carbon powder of the neostigmine group was higher than that of the normal control group,the difference was statistically significant(P<0.01);the propellant rates of carbon power of radices saussurease group Ⅰ,radices saussurease group Ⅱ and radices saussurease Ⅲ were lower than those of the neostigmine group obviously,the differences were statistically significant(P<0.05和P<0.01).The spontaneously contractile activity frequency and amplitude of the guinea-pig ileum smooth muscle in vitro after administration were higher than those before administration,the differences were statistically significant(P<0.05和P<0.01),and the amplitude increased with the dose of supercritical aqueous extract of radices saussurease increasing.Conclusion The supercritical aqueous extract of radices saussurease had the quality of promoting small intestinal movement of mice,had striking promotive effect on the inhibition of small intestine propulsion induced by adrenaline in mice,had the antagonistic effect on the hyperfunction of small intestine propulsion induced by neostigmine in mice and excited the spontaneous movement of mice ileum in vitro.