小鼠胎肝和骨髓造血基质细胞对粒系造血祖细胞增殖的影响

本文应用体外液体培养方法建立小鼠胎肝和骨髓造血基质细胞贴壁层,在贴壁细胞层上做粒系造血祖细胞集落(GM-CFU-C)培养,观察胎肝造血基质细胞和骨髓造血基质细胞对粒系造血祖细胞生长的影响。实验结果表明,培养1～2周的胎肝造血基质细胞贴壁层能抑制 GM-CFU-C 的集落生成,此抑制作用可被消炎痛拮抗。随贴壁培养时间的延长,胎肝造血基质细胞贴壁层转而增加 GM-CFU-C 产率。不同培养时间的骨髓造血基质细胞贴壁层均能明显地促进 GM-CFU-C 的生长。文中讨论了造血基质细胞与造血的关系以及两种来源造血基质细胞作用不同的可能原因。

EFFECTS OF MURINE FETAL LIVER AND MARROW STROMAL CELLS ON THE PROLIFERATION OF GM-CFU-C

Using murine fetal liver stromal cells and bone marrow stromal cells as feed layers,the effects of stromal cells on the proliferation of GM-CFU-C were investigated.It was found that the fetal liver stromal cells harvested at the early stage of culture had an inhibitory ef- fect on the growth of GM-CFU-C and this inhibition could be reversed by indomethacine. The bone marrow one exerted a significant stimulating effect on the proliferation of GM- CFU-C.The mechanism of the effects of fetal liver and marrow stromal cells on the growth of GM-CFU-C and the difference between them were discussed.