INHIBITORY EFFECT OF RETINOIC ACID ON EXPRESSION OF INDUCIBLE NITRIC OXIDE SYNTHASE GENE IN L929 CELLS |
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Authors: | Gi-Su Oh Hyun-Ock Pae Won-Gil Seo Min-Kyo Shin II-Kwang Kim Kyu-Yun Chai Young-Gab Yun Tae-Oh Kwon Joung-Sik Lim Hun-Taeg Chung |
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Affiliation: | a Medicinal Resources Research Center (MRRC), Wonkwang University School of Medicine, South Koreab Department of Urology, Wonkwang University School of Medicine, South Korea |
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Abstract: | Inflammation has been known to be associated with excess synthesis of nitric oxide (NO) by inducible NO synthase (iNOS). Retinoids have been reported to have anti-inflammatory activity, but the mechanism by which they can elicit this activity is poorly understood. The effects of retinoids on NO synthesis and iNOS gene expression in murine fibroblast L929 cells were examined. Treatment of the cells with interferon-γ resulted in excess NO synthesis and iNOS gene expression. All-trans-retinoic acid significantly inhibited NO synthesis and iNOS gene expression in a dose-dependent manner. Similarly, 9-cis-retinoic acid also inhibited NO synthesis, but retinol did not show any inhibitory effect on NO synthesis. These findings suggest that the modulation of iNOS gene expression is another possible pathway by which retinoids may function as anti-inflammatory agents. |
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Keywords: | Retinoic acid Nitric oxide Inducible nitric oxide synthase gene Inflammation L929 cells |
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