TT virus: preferential distribution in CD19(+) peripheral blood mononuclear cells and lack of viral integration. |
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Authors: | Qigui Yu Bruce Shiramizu Vivek R Nerurkar Ningjie Hu Cecilia M Shikuma Marian E Melish Karin Cascio Allison Imrie Yuanan Lu Richard Yanagihara |
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Affiliation: | Retrovirology Research Laboratory, Pacific Biomedical Research Center, University of Hawaii at Manoa, Honolulu, Hawaii, USA. qigui.yu@utoronto.ca |
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Abstract: | In order to determine whether peripheral blood mononuclear cells (PBMC) contain proviral DNA or double-stranded replicative forms of TT virus (TTV) and to define which cell subset harbors TTV, we analyzed 126 PBMC DNA samples by PCR, using the NG059/NG061/NG063 and the T801/T935 primer sets. TTV sequences were detected in nearly 20% (25/126) of PBMC DNA samples. Analysis of PBMC subsets revealed that TTV was preferentially distributed in CD19(+) cells. TTV DNA was also detected in CD3(+)/CD19(+) double-depleted cells but was undetectable in CD3(+) cells. After degradation of single-stranded DNA using mung bean nuclease (MBN), TTV could not be detected in previously TTV-positive PBMC DNA samples nor in DNA from CD19(+) cells, CD3(+)-depleted, and CD3(+)/CD19(+) double-depleted cells, indicating the lack of TTV DNA integration and the absence of viral double-stranded replicative intermediates in PBMC or PBMC subset cells. Our data indicate that PBMC and PBMC subsets are not the major sites of TTV replication. |
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Keywords: | TT virus replication peripheral blood mononuclear cell CD3+ cell CD19+ cell polymerase chain reaction circovirus |
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