转基因骨髓间充质干细胞复合纤维蛋白凝胶修复兔桡骨缺损

目的：探讨以复制缺陷重组腺病毒介导人骨形态发生蛋白(BMP)-2转基因的骨髓间充质干细胞(MSCs)复合纤维蛋白凝胶来修复节段性骨缺损的可行性。方法：于14只日本大耳白兔双侧桡骨中段造成10mm骨缺损，采用四种方法进行处理：A组植入转基因MSCs与纤维蛋白凝胶的复合物；B组植入单纯MSCs与纤维蛋白凝胶的复合物；C组植入纤维蛋白；D组不做处理，留作空白对照。每组7条桡骨。术后12周进行组织学、放射学检查及生物力学检测。结果：A组缺损区在成骨活跃程度、骨再生量和再生髓腔结构等方面均显著优于B组，其骨缺损得到了较彻底的修复。C、D两组均不能产生骨性愈合。结论：转基因MSCs复合纤维蛋白凝胶修复节段性骨缺损可达到较好的效果。

Repair of segmental radial defects with composite of human fibrinous gel and transgeneic MSCs

Objective To study the feasibility of composite of human fibrinous gel and transgeneic MSCs aiming at replicating adenoviruses-bone morphogenetic proteins-2 (Ad-BMP-2) in repair of segmental bone defects. Methods Bone defects for 10 mm were created on the bilateral radius of 14 Japanese big ear rabbits and treated with four kinds of implantations (seven radii per group), ie, composite of transgeneic MSCs and fibrinous gel (Group A), composite of MSCs and fibrinous gel (Group B),fibrinous gel alone (Group C) and nothing as control group. The defects were observed radiologically and histologically and examined by biomechanical test at the 12th week after operation. Results The defects treated in the Group A were repaired and regenerated much more new bone, bridged earlier and stronger than those in the Group B 12 weeks after operation. The defects treated in the Groups C and D could not attain osseous tissue healing. Conclusions The composite of transgeneic MSCs and fibrinous gel can be used to effectively repair segmental bone defects.