Preparation of monoclonal antibodies to chicken bone phosphoproteins |
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| Authors: | Kaoru Yamazaki Masanori Suzuki Yuko Mikuni-Takagaki Kiyotaka Hiraiwa Beatrice Lefteriou Melvin J Glimcher MD |
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| Institution: | (1) Laboratory for the Study of Skeletal Disorders, Department of Orthopedic Surgery, Children's Hospital and Harvard Medical School, 300 Longwood Avenue, 02115 Boston, Massachusetts, USA;(2) Present address: Department of Orthopedic Surgery, Hamamatsu University School of Medicine, Hamamatsu, Japan;(3) Present address: Tokushima University Dental School, Takushima, Japan |
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| Abstract: | Summary Monoclonal and polyclonal antibodies were raised against 150k, 60k, 32k, and 15k phosphoprotein components of 14-week chicken
bone. Hybridomas were prepared by immunizing Balb/c mice with 150k, 60k, or 32k phosphoproteins followed by fusion of their
spleen cells with X63-Ag8.653 myeloma cells. Polyclonal antibodies to the 60k, 32k, and 15k phosphoprotein components were
produced in immunized rabbits. Immunological cross-reactivity between antigen and antibody was examined by enzyme-linked immunosorbent
assay (ELISA) and dot-blotting. There was cross-reactivity between the 150k, 32k, and 15k phosphoprotein components and between
the 150k, 90K, and 60k components. The antibodies raised against 60k component do not bind 32k and 15k antigens. |
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| Keywords: | Phosphoproteins Monoclonal antibody Bone Cross-reactivity |
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