| 分子标记及其在生物遗传多样性研究中的应用 |
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| 引用本文： | 朱新产,王宝维,张庭荣. 分子标记及其在生物遗传多样性研究中的应用[J]. 中国医学生物技术应用, 2002, 0(2) |
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| 作者姓名： | 朱新产 王宝维 张庭荣 |
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| 作者单位： | 莱阳农学院动科系分子生物学研究室 莱阳265200(朱新产，王宝维)，莱阳农学院动科系分子生物学研究室 莱阳265200(张庭荣) |
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| 基金项目： | 山东省教育厅基金(J01S08)，莱阳农学院基金(000-3) |
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| 摘 要： | 遗传标记(源于DNA序列差异的特征)可以通过很多不同的途径显示出来。随着生化与分子生物技术的发展完善,可以从分子水平上直接显示它的变化-分子标记。分子标记技术具有可靠性,高效性, 因而被广泛应用于多种研究领域。分子标记通常包括同工酶(或蛋白质)和DNA标记。本文综述生化和DNA标记(蛋白/酶,RFLP, RAPD, AFLP, SSR,SNP, SSLP等)技术的原理、发展及应用。
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| 关 键 词： | 生物遗传多样性 分子标记 PCR |
Molecular Markers and their Application in the Study of Organism Genetic Diversity |
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| Xinchan Zhu,Baowei Wang,Tingrong Zhang. Molecular Markers and their Application in the Study of Organism Genetic Diversity[J]. The Chinese Academic Medical Magazine of Organisms, 2002, 0(2) |
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| Authors: | Xinchan Zhu Baowei Wang Tingrong Zhang |
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| Abstract: | Genetic markers, derived from differences in the DNA sequence , can be observed in several different way.With the improvement of biochemistry and molecular biology techniques it can directly be detected at the molecularlevel. That is the molecular marker techniques. They have been widely used in many research fields due to their reliabil-ity and efficiency. The molecular marker, in general, include Isozymes(or protein)markers and DNA markers. Thispaper mainly interpret biochemistry markers and DNA markers, such as RFLP, RAPD, AFLP, SSR, SNP and SSLP etc,and briefly outline the basic principles of the techniques, their advantages/disadvantages and application. |
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| Keywords: | organism genetic diversity molecular markers Southern blotting PCR |
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