用细胞转化实验检测S江有机污染致癌危险性

用Ｓ江冬夏两季的江水有机提取物分别做ＮＩＨ３Ｔ３细胞转化试验。结果表明：江水有机提取物各剂量组均能诱发该细胞出现典型的细胞转化灶,其细胞转化率呈现剂量－－反应关系。转化细胞的恶性表现是生长失去接触性抑制并能在软琼脂内生长。进一步提示了Ｓ江有机污染有潜在的致癌危险性。

EVALUATION ON CARCINOGENIC RISK FROM ORGANIC POLLUTION IN THE S RIVER BY CELL TRANSFORMATION

NIH3T3 cell line was used for the study transformation induction by organic extracts from water in the S river. The result indicated that the malignant transformed foci were induced by organic extracts mentioned above and the cellular transformation frequencies presented a dose response relationship and determination of malignant cells disappeared in restraining of contact and grew in culture of soft agar while control cells did not. Thus, the present study demonstrates further that organic pollution from water in the S river has the potential risk of carcinogencity.