应用LSCM对脑脊液淋巴白血病细胞内质网的研究

目的 应用激光扫描共聚焦显微镜（LSCM)技术对脑脊液淋巴白血病细胞内质网进行立体、动态、定量研究。方法 将所观察脑脊液细胞分为正常淋巴细胞组、急性淋巴白血病细胞组、鞘内注射治疗后急性淋巴白血病细胞组。以内质网荧光探针DioC6(3)标记后，得到内质网的断层扫描图像、荧光强度地形立体分布图，并且测量内质网的荧光强度值。结果 急性淋巴白血病细胞组与正常淋巴细胞组、鞘内注射治疗后淋巴白血病细胞组相比，内质网空间立体分布有明显差别，平均荧光值有非常显著性差异(P＜0．01)。结论 LSCM对中枢神经系统淋巴白血病的诊断、鉴别诊断、细胞分化程度和功能状态判断有一定意义。

Study on endoplasmic reticulum in cerebrospinal fluid leukemia cells by LSCM

Objective To investigate three-dimensionally, dynamically and quantitatively the endoplasmic reticulum of cerebrospinal fluid leukemia cells by laser scanning confocal microscopy (LSCM).Methods Endoplasmic reticulum of all lymphocytes (normal lymphocytes, leukemia cells and cured leukemia cells) was stained with fluorescent probe DioC 6(3), sectional scanograph and fluorescent intensity stereophotography were obtained and finally fluorescent values of cellular endoplasmic reticulum were individually measured.Results Compared with normal lymphocyte and cured leukemia cells, endoplasmic reticulum three-dimensional character of leukemia cells was varied and fluorescent value differences were sigificant (P< 0.01).Conclusions The LSCM above may be useful for central nervous system leukemia diagnosis, differential diagnosis, judgement of cells differentiation level and function.