Evaluation of automated sex hormone binding globulin immunoassays |
| |
Authors: | Jin Ming Wener Mark H Bankson Daniel D |
| |
Institution: | Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA. |
| |
Abstract: | BACKGROUND: Sex hormone binding globulin (SHBG) is an important regulator of testosterone and estradiol. STUDY DESIGN: We validated the Diagnostic Products Corporation (DPC) and Roche Diagnostic SHBG immunoassays on the DPC Immulite 2000 and Roche Modular E170 analyzers. RESULTS: The coefficient of variation for SHBG kits from both manufacturers was in the range of 3.9-7.7% (between-run) and 0.95-5.0% (within-run), free of interference from hemoglobin, bilirubin, lipid, and rheumatoid factor, and linear up to at least 170 nM SHBG. The results of the two methods, however, were biased by up to 29% depending on the SHBG concentration. CONCLUSION: The SHBG assays perform well but standardization is needed. |
| |
Keywords: | SHBG Sex hormone binding globulin Sex steroid binding globulin SSBG Testosterone Free testosterone Bioavailable testosterone Method comparison |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|