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| 2 型糖尿病患者线粒体常见基因突变PCR检测的局限性 | |
| 引用本文： | 刘建伟,詹志伟,王磊,王欣然,崔志辉,吴多彬,刘静,刘玲玲,王士雯,叶玲. 2 型糖尿病患者线粒体常见基因突变PCR检测的局限性[J]. 军医进修学院学报, 2006, 27(1): 25-27 |
| 作者姓名： | 刘建伟 詹志伟 王磊 王欣然 崔志辉 吴多彬 刘静 刘玲玲 王士雯 叶玲 |
| 作者单位： | 1. 解放军总医院,老年医学研究所,北京,100853 2. 解放军第二炮兵总医院,北京,100088 3. 解放军总医院,老年心血管病研究所,北京,100853 |
| 摘 要： | 目的:研究北京地区汉族人群中线粒体DNA(m itochondrial DNA,m tDNA)多位点突变频率与2型糖尿病之间的相关性。方法:采用直接PCR、PCR-限制性片段长度多态性(restriction fragm ent length polymorph ism,RFLP)、PCR-时相温度梯度凝胶电泳(temporal temperature grad ient gel electrophoresis,TTGE)法筛查m tDNA 4977片段缺失、线粒体tRNALeu(UUR)A3243G突变和m tDNA T14577C突变。结果:在250例2型糖尿病患者及142例健康对照外周血基因组总DNA中,未发现m tDNA 4977缺失、线粒体tRNALeu(UUR)A3243G突变及m tDNA T14577C突变。结论:m tDNA 4977缺失、线粒体tRNALeu(UUR)A3243G突变及m tDNA T14577C突变在外周血中没有检出,采用常规PCR法检测2型糖尿病患者外周血线粒体DNA突变具有较大的局限性。 |
| 关 键 词： | 糖尿病 非胰岛素依赖型 DNA线粒体 突变 遗传学 |
| 文章编号： | 1005-1139（2006）01-0025-03 |
| 收稿时间： | 2005-03-08 |
| 修稿时间： | 2005-04-14 |
Limitation on detecting common gene mutations of mitochondrial DNA in type 2 diabetes with PCR |
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| LIU Jian-wei,ZHAN Zhi-wei,WANG Lei,WANG Xin-ran,CUI Zhi-hui,WU Duo-bin,LIU Jing,LIU Ling-ling,WANG Shi-wen,YE Ling. Limitation on detecting common gene mutations of mitochondrial DNA in type 2 diabetes with PCR[J]. Academic Journal of Pla Postgraduate Medical School, 2006, 27(1): 25-27 | |
| Authors: | LIU Jian-wei ZHAN Zhi-wei WANG Lei WANG Xin-ran CUI Zhi-hui WU Duo-bin LIU Jing LIU Ling-ling WANG Shi-wen YE Ling |
| Affiliation: | 1. Institute of Geriatrics, PLA General Hospital, Beijing 100853, China; 2. Second Artillery General Hospital, Beijing 100088, China; 3. Institute of Geriatric Cardiology, PLA General Hospital, Beijing 100853, China |
| Abstract: | Objective:To study the association of common gene mutations of mitochondrial DNA(mtDNA) in patients with type 2 diabetes mellitus(DM2).Methods: Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) and Polymerase chain reaction-temporal temperature gradient gel electrophoresis(PCR-TTGE) were carried out to screen the fragment deletion of mtDNA 4977 and the mutations of tRNA ~(Leu(UUR))A 3243G and mtDNA T14577C.Results:Fragment deletion of mtDNA 4977,mutations of tRNA ~(Leu(UUR))A3243G and mtDNA T14577C were not found neither in DM2 nor healthy controls.Conclusion:There is a limitation on detecting common gene mutations of mitochondrial DNA in type 2 diabetes by PCR for the heterogenicity of mtDNA in the blood samples. |
| Keywords: | diabetes mellitus non-insulin-dependent DNA mitochondrial mutation genetics |
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