反相高效液相色谱法测定肾移植术后全血环孢素A浓度

目的建立单步萃取反相高效液相色谱法测定人全血中环孢素A(CsA)浓度。方法患者血样经乙醚单步萃取,环孢素D(CsD)为内标,C18色谱柱,乙腈-水(80:20)为流动相,流速1.0ml/min,柱温70℃,检测波长214nm。结果CsA全血浓度在50～600ng/ml范围内线性关系良好,r=0.9998,最低检测浓度为20ng/ml,平均回收率100.44%,日内和日间RSD均低于5%。结论反相高效液相色谱法简便灵敏、准确可靠、经济,可用于临床人体器官移植术后全血中CsA浓度的监测。

Determinaton of cyclosporin A concentration of whole blood in patients after renal transplantation by RP-HPLC method

Objective To establish a method of RP -HPLC for determination of cyclosporin A（CsA） of whole blood in patients, after renal transplantation by single-step extraction. Methods CsA was extracted from whole blood with diethyl ether and separated using a C18 column, cyclosporin D was used as internal standard. The mobil phase was composed of acetonitrile-water （80：20）, the flow rate was 1.0ml/min, the temperature was maintained at 70℃and the detecting wavelength was 214nm. Results The assay was shown to produce linear calibration curves over the range from 50 to 600ng/ml for cyclosporine, r=-0.9998. The lowest plasma limitation was 20ng/ml.The average recovery was 100.44%,and RSD in a day and between days did not exceed 5%. Conclusion The method is found to be convenient, accurate and economical for determination of cyclosporine A of whole blood in patients after renal transplantation.