补阳还五汤促进带蒂皮瓣血供建立及提前断蒂的实验研究

目的探讨补阳还五汤促进带蒂皮瓣血供建立的机制及对断蒂时间的影响。方法新西兰家兔64只,随机分成实验组和对照组各32只,又按断蒂时间不同分为2日断蒂组、3日断蒂组、4日断蒂组、5日断蒂组,实验组予以补阳还五汤灌服。观察实验组与对照组不同断蒂时间的皮瓣成活率、皮瓣组织大体观察、苏木精和伊红(HE)染色法观察血管生长情况并计数,并进行比较。结果实验组第2、3、4日断蒂组皮瓣成活率比对照组明显提高(P<0.01,P<0.05),2组5日断蒂组皮瓣存活率比较差异无统计学意义(P>0.05)。大体观察实验组情况优于对照组,2组皮瓣新生血管计数比较差异有统计学意义(P<0.05,P<0.01)。结论补阳还五汤对家兔带蒂皮瓣具有明显促进血供建立、提高皮瓣成活率及缩短断蒂时间的作用,可能对临床应用具有指导意义。

Influence of Buyanghuanwu decoction on improving blood supply of pedicled skin flap and time of pedicle division

Objective To evaluate the influence of Buyanghuanwu decoction to improve blood supply of pedicled skin flap and time of pedicle division. Methods One hundred and Sixty-four New Zealand rabbits were randomly divided into experimentgr oup and control group. Rabbits in each group were divided into 2,3,4,5 days to pedicle division. Rabbits in experiment group administrated by Buyanghuanwu decoction. Survival rate of pedicle flap in different time of pedicle division and histology of pedicle flap were measured. Development of blood vessel was observed and counted by staining method of H and E. Results Survival rate in 2,3,4 days to pedicle division in experiment group were higher than those in control group (P<0.01,P<0.05). Status of histology of pedicle flap and development of blood vessel in experiment group were superior to those in control group (P<0.05,P<0.01). Conclusion Buyanghuanwu decoction might play an important role in improvement the survival of pedicle flap on rabbit with early pedicle division.