灵芝多糖对小鼠腹腔巨噬细胞胞浆游离Ca2+浓度的影响

 目的:探讨灵芝多糖(GLP)对免疫细胞信号转导过程的影响?方法:采用激光扫描共聚焦显微镜(LSCM)技术,动态监测GLP均一体组分GLB₇对小鼠腹腔巨噬细胞(MΦ)胞浆游离Ca²⁺浓度(Ca²⁺]i)的影响?结果:GLB₇(20μg·ml^-1)引起小鼠腹腔MΦ中Ca²⁺]i明显升高,升高值为(248±18)%(n=6);GLB₇引起小鼠腹腔MΦ外钙内流及MΦ内IP₃敏感和IP₃非敏感钙池释放Ca²⁺,Ca²⁺]i升高值分别为(76±10)%,(58±10)%,(41±8)%(n=6);GLB₇对MΦCa²⁺]i的影响与K⁺通道及其引起的膜电位变化无关?结论:MΦ是灵芝多糖作用的靶细胞;GLB₇引起MΦ中Ca²⁺]i快速升高,可能是灵芝多糖产生作用的重要途径?

Effect of Ganoderma polysacchride on intracellular free calcium in murine peritoneal macrophages

OBJECTIVE:To investigate the action of the Ganoderma polysacchride on intracellular signal events in immunocytes.METHODS:Laser scanning confocal microscope imaging of the calcium fluorescent indicator dye Fluo3/AM was used to determine the kinetic changes of Ca²⁺]i in murine peritoneal macrophages induced by a Ganoderma polysacchride,designated GLB₇.RESULTS:It was found that GLB₇(20μg·ml^-1) could increase Ca²⁺]i in murine peritoneal macrophages,the average percentage of increase in Ca²⁺]i was (248±18)%(n=6).The increase in Ca²⁺]i induced by GLB₇was due to the influx of extriacellular Ca²⁺ and intracellular Ca²⁺ release through both IP₃-sensitive and IP₃-insensitive Ca²⁺ stores,deplarization in macrophage membrane did not affect the increase in Ca²⁺]i.CONCLUSION:Macrophage is one of the target cells of Ganderma polysacchrides.The stimulation of the increase in Ca²⁺]i may be an important channel for the polysacchrides to achieve their pharmacological actions